Rabbit Polyclonal HEY1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HEY1 aa 1 to C-terminus.
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- Acta neuropathologica communications 11:1982023A novel patient-derived meningioma spheroid model as a tool to study and treat epithelial-to-mesenchymal transition (EMT) in meningiomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurien L van de Weijer,Emanuela Ercolano,Ting Zhang,Maryam Shah,Matthew C Banton,Juri Na,Claire L Adams,David Hilton,Kathreena M Kurian,C Oliver HanemannPubMed 38102708
- Molecular carcinogenesis 63:22-332023Single-cell sequencing reveals SATB2/NOTCH1 signaling promotes the progression of malignancy of epithelial cells from papillary thyroid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqian Zhang,Jing Wang,Dongning Huang,Zhu Liu,Tie Lu,Chen Cui,Zhendong LiPubMed 37877736
- Cells 11:2022HOCI Probe CPP Induces the Differentiation of Human Dermal Fibroblasts into Vascular Endothelial Cells through PHD2/HIF-1α/HEY1 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoling Cui,Jie Wen,Nan Li,Xuxiao Hao,Shangli Zhang,Baoxiang Zhao,Xunwei Wu,Junying MiaoPubMed 36231088
- Cell stem cell 29:1402-1419.e82022Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zheng,Robin Zhexuan Yan,Shiyu Sun,Mutsumi Kobayashi,Lifeng Xiang,Ran Yang,Alexander Goedel,Yu Kang,Xufeng Xue,Sajedeh Nasr Esfahani,Yue Liu,Agnes M Resto Irizarry,Weisheng Wu,Yunxiu Li,Weizhi Ji,Yuyu Niu,Kenneth R Chien,Tianqing Li,Toshihiro Shioda,Jianping FuPubMed 36055194
- Thoracic cancer 13:1961-19732022Galectin-3 enhances trastuzumab resistance by regulating cancer malignancy and stemness in HER2-positive breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqiu Chen,Jiawei Xu,Wang Pan,Xiaofan Xu,Xueping Ma,Ya'nan Chu,Lu Wang,Shuyun Pang,Yujiao Li,Bingjie Zou,Guohua Zhou,Jun GuPubMed 35599381
- Nature communications 13:15372022GIT1 protects against breast cancer growth through negative regulation of Notch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSongbai Zhang,Ayako Miyakawa,Malin Wickström,Cecilia Dyberg,Lauri Louhivuori,Manuel Varas-Godoy,Kati Kemppainen,Shigeaki Kanatani,Dagmara Kaczynska,Ivar Dehnisch Ellström,Lotta Elfman,Pauliina Kronqvist,Heli Repo,Katsuhiko Mikoshiba,Cecilia Sahlgren,John Inge Johnsen,Per UhlénPubMed 35318302
- Journal of materials science. Materials in medicine 33:42021Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou-Shan Tao,Tian-Lin Li,Hong-Guang Xu,Min YangPubMed 34940936
- Bioengineered 12:10878-108902021Cytochrome P450 2J2 inhibits the proliferation and angiogenesis of retinal vascular endothelial cells by regulating the Notch signaling pathway in a hypoxia-induced retinopathy model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang,Qi Xiong,Lin Yang,Yanni Xue,Min Ke,Zhi LiPubMed 34666595
- Molecular medicine (Cambridge, Mass.) 27:1032021Increased hemoglobin and heme in MALDI-TOF MS analysis induce ferroptosis and promote degeneration of herniated human nucleus pulposus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Shan,Ximing Xu,Jing Zhang,Peng Cai,Han Gao,Yingjie Lu,Jiangang Shi,Yinlong Guo,Yue SuPubMed 34496740
- European journal of pharmacology 906:1742682021Intermittent administration sodium valproate has a protective effect on bone health in ovariectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou-Shan Tao,Wan-Shu Zhou,Hong-Guang Xu,Min YangPubMed 34166702
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HEY1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5J3
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (PubMed:11095750). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3 (PubMed:15485867). Involved in the regulation of liver cancer cells self-renewal (PubMed:25985737).
Alternative names
BHLHB31, CHF2, HERP2, HESR1, HRT1, HEY1, Hairy/enhancer-of-split related with YRPW motif protein 1, Cardiovascular helix-loop-helix factor 2, Class B basic helix-loop-helix protein 31, HES-related repressor protein 1, Hairy and enhancer of split-related protein 1, Hairy-related transcription factor 1, CHF-2, bHLHb31, HESR-1, HRT-1, hHRT1
Recommended products
Rabbit Polyclonal HEY1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 23 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HEY1 aa 1 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HEY1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5J3
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HEY1 protein also known as Hes-related family bHLH transcription factor with YRPW motif 1 plays a significant role as a transcriptional repressor. It weighs approximately 33 kilodaltons. HEY1 is part of the hairy and enhancer of split family of proteins. Expression of HEY1 occurs in various tissues including the heart brain and liver. Within cells HEY1 localizes to the nucleus where it modulates gene expression by binding to specific DNA sequences.
- Biological function summary
HEY1 functions as an important regulator of developmental processes. It participates in the Notch signaling pathway and regulates the transcription of genes involved in cell fate decisions. HEY1 forms a part of transcriptional complexes often associating with other proteins to modulate expression patterns important for differentiation and proliferation. These complexes enable it to exert control over target gene expression influencing processes like angiogenesis and myogenesis.
- Pathways
HEY1 is prominently involved in the Notch signaling and Wnt pathways. Notch signaling regulates cell communication important for cell differentiation while the Wnt pathway influences cell growth and development. In both pathways HEY1 modulates signal transduction by interacting with proteins like RBPJ in the Notch pathway. HEY1's role in these pathways reveals its importance in maintaining cellular architecture and instructive developmental cues.
- Associated diseases and disorders
HEY1 is associated with various cancers and cardiovascular diseases. Abnormal HEY1 expression correlates with tumorigenesis in cancers such as osteosarcoma where it may interact with proteins like HES1 to influence tumor growth. Additionally HEY1's involvement in cardiovascular diseases links to its role in regulating vascular remodeling and heart development where an imbalance in HEY1 expression might contribute to pathological outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-HEY1 antibody (ab154077)
12% SDS PAGE
All lanes: Western blot - Anti-HEY1 antibody (ab154077) at 1/1000 dilution
Lane 1: A549 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HCT116 whole cell lysate at 30 µg
Lane 4: MCF-7 whole cell lysate at 30 µg
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (ab154077)
ab154077 staining HEY1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/2000) (green). Alpha tubulin stained red and nuclei stained blue
Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (ab154077)
ab154077 staining HEY1 in H1299 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (1/1000) (green). Nuclei were stained blue with Hoechst 33342
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-HEY1 antibody (ab154077)
HEY1 western blot using anti-HEY1 antibody ab154077. Publication image and figure legend from Jiang, S., Zhou, F., et al., 2020, J Cancer, PubMed 32284759.
ab154077 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154077 please see the product overview.
HEY1 is a key factor in the tumorigenicity of 143B osteosarcoma cells. (A) Protein expression level of HEY1 in six osteosarcoma cell lines (MG-63, Saos-2, U-2 OS, MNNG/HOS, 143B, and SJSA-1). (B) Protein expression level of HEY1 after lentivirus-mediated RNA interference in 143B osteosarcoma cells. (C) Lentivirus infection efficiency detected by fluorescence microscopy (scale bar = 200 μm). (D) Lentivirus-infected 143B osteosarcoma cell pellet under visible light. (E) Images of 143B cell-bearing mice under 530-nm laser irradiation at the experimental endpoint. Subcutaneous tumors at the experimental endpoint (F), tumor growth curves (G), and tumor weight (H) are shown. (I) Protein expression level of HEY1 in Saos-2 and HEY1-overexpressed Saos-2 cells. (J) Tumorigenicity assay of Saos-2 cells after HEY1-overexpression. Subcutaneous tumors at the experimental endpoint (K), tumor growth curves (L), and tumor weight (M) of Saos-2 cells after HEY1-overexpression are shown.
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