JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317210

Anti-HEY1 antibody [HL2367]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal HEY1 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Rat, Zebrafish, Dog, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human HEY1.

View Alternative Names

BHLHB31, CHF2, HERP2, HESR1, HRT1, HEY1, Hairy/enhancer-of-split related with YRPW motif protein 1, Cardiovascular helix-loop-helix factor 2, Class B basic helix-loop-helix protein 31, HES-related repressor protein 1, Hairy and enhancer of split-related protein 1, Hairy-related transcription factor 1, CHF-2, bHLHb31, HESR-1, HRT-1, hHRT1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEY1 antibody [HL2367] (AB317210)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEY1 antibody [HL2367] (AB317210)

ab317210 detects HEY1 protein at nucleus by immunohistochemical analysis. Sample : Paraffin-embedded mouse lung. HEY1 stained by ab317210 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)
  • WB

Supplier Data

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317210 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HEY1 antibody [HL2367] (ab317210) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

PC-3 whole cell extracts at 30 µg

Lane 3:

A549 whole cell extracts at 30 µg

Lane 4:

K562 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)
  • WB

Supplier Data

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)

Rat tissue extract (50 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317210 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HEY1 antibody [HL2367] (ab317210) at 1/1000 dilution

All lanes:

Rat lung tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)
  • WB

Supplier Data

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317210 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HEY1 antibody [HL2367] (ab317210) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged HEY1 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)
  • WB

Supplier Data

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)

Whole cell extract (30 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317210 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-HEY1 antibody [HL2367] (ab317210) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)
  • WB

Supplier Data

Western blot - Anti-HEY1 antibody [HL2367] (AB317210)

Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317210 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HEY1 antibody [HL2367] (ab317210) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2367

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Dog, Zebrafish

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HEY1.

Q9Y5J3

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HEY1 protein also known as Hes-related family bHLH transcription factor with YRPW motif 1 plays a significant role as a transcriptional repressor. It weighs approximately 33 kilodaltons. HEY1 is part of the hairy and enhancer of split family of proteins. Expression of HEY1 occurs in various tissues including the heart brain and liver. Within cells HEY1 localizes to the nucleus where it modulates gene expression by binding to specific DNA sequences.
Biological function summary

HEY1 functions as an important regulator of developmental processes. It participates in the Notch signaling pathway and regulates the transcription of genes involved in cell fate decisions. HEY1 forms a part of transcriptional complexes often associating with other proteins to modulate expression patterns important for differentiation and proliferation. These complexes enable it to exert control over target gene expression influencing processes like angiogenesis and myogenesis.

Pathways

HEY1 is prominently involved in the Notch signaling and Wnt pathways. Notch signaling regulates cell communication important for cell differentiation while the Wnt pathway influences cell growth and development. In both pathways HEY1 modulates signal transduction by interacting with proteins like RBPJ in the Notch pathway. HEY1's role in these pathways reveals its importance in maintaining cellular architecture and instructive developmental cues.

HEY1 is associated with various cancers and cardiovascular diseases. Abnormal HEY1 expression correlates with tumorigenesis in cancers such as osteosarcoma where it may interact with proteins like HES1 to influence tumor growth. Additionally HEY1's involvement in cardiovascular diseases links to its role in regulating vascular remodeling and heart development where an imbalance in HEY1 expression might contribute to pathological outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (PubMed : 11095750). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3 (PubMed : 15485867). Involved in the regulation of liver cancer cells self-renewal (PubMed : 25985737).
See full target information HEY1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com