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AB221931

Anti-HEY2 antibody

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(2 Publications)

Rabbit Polyclonal HEY2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HEY2 aa 1-100.

View Alternative Names

BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, HEY2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, Hairy-related transcription factor 2, Protein gridlock homolog, hCHF1, bHLHb32, HESR-2, HRT-2, hHRT2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEY2 antibody (AB221931)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEY2 antibody (AB221931)

PFA-fixed, Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells stained for HEY2 (green) using ab221931 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HEY2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UBP5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'HEY2' also known as 'heart and neural crest derivatives-expressed protein 2' or simply 'HEY’ or 'heytwo' is a transcription factor from the hairy and enhancer of split (HES) family. This protein has a molecular mass of approximately 37 kDa. It plays a significant role in the regulation of gene expression by binding to specific sequences in DNA. 'HEY2' gets expressed in different tissues particularly in heart vascular and neural crest-derived cells. Its expression appears during various stages of development and remains present in certain adult tissues underlining its continuing importance in cellular function.
Biological function summary

'HEY2' participates in the regulation of cell differentiation and proliferation. It functions within the Notch signaling pathway and often associates with transcriptional repression complexes. 'HEY2' has the ability to modulate the activity of other transcription factors by influencing their binding efficiency. Through these interactions it helps establish cellular identities and maintain the balance between cell populations within developing tissues. Its involvement in cardiovascular development among others stands as a testament to its functional diversity.

Pathways

This protein occupies an important position in the Notch signaling pathway and the TGF-beta signaling pathway. Within the Notch pathway 'HEY2' functions as a downstream effector that transduces signals necessary for cell fate determination. It is related closely to other Notch signaling components like JAG1 and DLL4. Additionally the interaction with the TGF-beta signaling pathway provides a link to cellular processes like apoptosis and epithelial-mesenchymal transition although the precise mechanisms demand further exploration.

Mutations and aberrant expression levels of 'HEY2' have been associated with congenital heart defects and cardiomyopathies. Its involvement with congenital heart defects is often observed with other proteins in the Notch pathway such as NOTCH1. Researchers find connections between 'HEY2' expressions and specific cardiovascular pathologies indicating its potential as a target for therapeutic interventions. Involvement in these conditions emphasizes the necessity for more detailed studies of this protein's behavior under pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
See full target information HEY2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Glia 71:2720-2734 PubMed37522284

2023

Zinc finger protein 335 mediates lipopolysaccharide-induced neurodegeneration and memory loss as a transcriptional factor in microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Kong,Zhen Xie,Xiaokang Shang,Yoshinori Hayashi,Fei Lan, Narengaowa,Shuxuan Zhao,Hui Li,Zhenzhen Quan,Zhou Wu,Hiroshi Nakanishi,Hong Qing,Junjun Ni

Cells 9: PubMed32260159

2020

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Applications

Unspecified application

Species

Unspecified reactive species

Anton G Kutikhin,Alexey E Tupikin,Vera G Matveeva,Daria K Shishkova,Larisa V Antonova,Marsel R Kabilov,Elena A Velikanova
View all publications
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Product promise

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