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AB323267

Anti-HEY2 antibody [EPR29024-13]

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Rabbit Recombinant Monoclonal HEY2 antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

BHLHB32, CHF1, GRL, HERP, HERP1, HRT2, HEY2, Hairy/enhancer-of-split related with YRPW motif protein 2, Cardiovascular helix-loop-helix factor 1, Class B basic helix-loop-helix protein 32, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, Hairy-related transcription factor 2, Protein gridlock homolog, hCHF1, bHLHb32, HESR-2, HRT-2, hHRT2, Chf1, Herp, Herp1, Hesr2, Hrt2, Hairy/enhancer-of-split related with YRPW motif protein 2, HES-related repressor protein 2, Hairy and enhancer of split-related protein 2, Hairy-related transcription factor 2, Protein gridlock homolog, HESR-2, HRT-2, mHRT2

3 Images
Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 2:

HEK-293 transfected with siRNA specifically targeting HEY2 whole cell lysate at 80 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Exposure time: 70s

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 48 seconds, lanes 3-4 : 180 seconds

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

Untreated HEK-293 whole cell lysate at 80 µg

Lane 2:

HEK-293 treated with 10 µM MG-132 for 6 hours whole cell lysate at 80 µg

Lane 3:

Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

Lane 4:

Neuro-2a treated with 10 µM MG-132 for 16 hours whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)
  • WB

Supplier Data

Western blot - Anti-HEY2 antibody [EPR29024-13] (AB323267)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HEY1 and HEYL.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - Total Protein Control ChIP Grade (ab213204) at 1/5000 dilution.

All lanes:

Western blot - Anti-HEY2 antibody [EPR29024-13] (ab323267) at 1/1000 dilution

Lane 1:

His-tagged human HEY2 recombinant protein at 10 ng

Lane 2:

His-tagged human HEY1 recombinant protein at 10 ng

Lane 3:

His-tagged human HEYL recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,90 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29024-13

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.
See full target information HEY2

Additional targets

Hey2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com