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AB225992

Anti-HFBI antibody

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(2 Publications)

Rabbit Polyclonal HFBI antibody. Suitable for WB and reacts with Recombinant full length protein - Trichoderma reesei samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Trichoderma reesei hfb1.

View Alternative Names

Hydrophobin-1, Hydrophobin I, HFBI, hfb1

1 Images
Western blot - Anti-HFBI antibody (AB225992)
  • WB

Supplier Data

Western blot - Anti-HFBI antibody (AB225992)

All lanes:

Western blot - Anti-HFBI antibody (ab225992) at 1/500 dilution

Lane 1:

Recombinant Hypocrea jecorina HFBI protein 100 ng

Lane 2:

Recombinant Hypocrea jecorina HFBI protein 50 ng

Lane 3:

Recombinant Hypocrea jecorina HFBI protein 25 ng

Lane 4:

Recombinant Hypocrea jecorina HFBI protein 12.5 ng

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Trichoderma reesei

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Trichoderma reesei hfb1.

P52754

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Trichoderma reesei": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p>Only tested with recombinant protein. No data available for endogenous protein.</p>" }, "Trichoderma reesei": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HFBI also known as hydrophobin I is a small protein with a molecular mass of approximately 7-9 kDa. It occurs in certain fungal species where it plays a role in forming hydrophobic surfaces. In certain fungi this protein expresses on both the cell wall and secreted materials. HFBI creates a water-repellent layer that facilitates the interactions between the fungus and its environment contributing to processes like spore dispersal and fungal growth.
Biological function summary

HFBI functions in forming membranes that involve interactions with hydrophobic structures. It does not occur as part of a larger complex but instead acts independently or in a coordination with other hydrophobins. Its ability to self-assemble into structures with surface activity allows it to contribute to the defense of the fungus by providing a protective barrier. This surface modification has significant implications for the adaptation and survival of fungi in various environments.

Pathways

HFBI contributes to processes like surface adhesion and developmental pathways unique to fungal species. While it is not directly involved in major mammalian signaling pathways it can be connected to processes involving cell wall biosynthesis and interactions related to other hydrophobins such as HFBII. These pathways reflect its roles in maintaining fungal cell structure and forming protective hydrophobic layers which are important for fungal physiology.

HFBI relates to pathologies in humans mainly through its involvement in certain fungal infections. Pathogenic fungi that express HFBI like Trichoderma species may cause diseases in immunocompromised individuals. During these infections HFBI helps form robust protective barriers hindering immune system access. HFBI interacts with other fungal proteins potentially including enzymes like chitinase that are important during the disease progression by destabilizing host tissues. Understanding HFBI's role in these fungi may help develop better strategies for managing fungal infections.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Contributes to surface hydrophobicity, which is important for processes such as association of hyphae in reproductive structures, dispersal of aerial spores and adhesion of pathogens to host structures.
See full target information hfb1

Publications (2)

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International journal of molecular sciences 22: PubMed33800567

2021

Comparative Study of Structural Changes of Polylactide and Poly(ethylene terephthalate) in the Presence of .

Applications

Unspecified application

Species

Unspecified reactive species

Grażyna B Dąbrowska,Zuzanna Garstecka,Ewa Olewnik-Kruszkowska,Grażyna Szczepańska,Maciej Ostrowski,Agnieszka Mierek-Adamska

OncoTargets and therapy 13:2399-2409 PubMed32273713

2020

CircRNA ZNF609 Knockdown Suppresses Cell Growth via Modulating miR-188/ELF2 Axis in Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyan Li,Yujie Li,Min Yu
View all publications

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