AB170912

Anti-HFE antibody [EPR6750]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal HFE antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HLAH, HFE, Hereditary hemochromatosis protein, HLA-H

1 Images

Lab

Western blot - Anti-HFE antibody [EPR6750] (AB170912)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 1% BSA/TBST.

All lanes:

Western blot - Anti-HFE antibody [EPR6750] (ab170912) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

A375 (Human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6750

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HFE protein also known as the hemochromatosis protein or HFE 5000 is a critical component in the regulation of iron absorption in the body. Structurally this protein has a known mass of about 50 kDa and is expressed primarily in the liver pancreas and intestines. The HFE belongs to the class I major histocompatibility complex (MHC) proteins. Its main function is to interact with transferrin receptors regulating the amount of iron that's absorbed from the diet into the bloodstream.

Biological function summary

This protein plays a significant role in maintaining iron homeostasis within the body. HFE does not function alone; it forms a complex with transferrin receptors to modulate iron uptake. Its interaction with the transferrin-bound iron helps prevent excess iron build-up by reducing the binding affinity of transferrin for its receptor. Through these interactions HFE balances iron storage and transport ensuring proper iron levels in vital organs.

Pathways

HFE is an integral part of iron metabolism and homeostasis pathways. It closely interacts with proteins like TFR1 (Transferrin Receptor 1) in these pathways. The hepcidin pathway also involves HFE where it indirectly influences the synthesis of hepcidin by the liver. Hepcidin plays an important role in preventing iron overload by binding and degrading the iron export protein ferroportin hence regulating the release of iron from cells adding a layer of control over iron homeostasis.

HFE mutations can lead to hereditary hemochromatosis a condition characterized by excessive iron accumulation in tissues which leads to organ damage. A common mutation is the C282Y mutation which disrupts the protein's normal function. This disorder links to other proteins related to iron metabolism such as hepcidin. Proper functioning of HFE is essential to avoid diseases like hemochromatosis as its impairment results in misregulated iron levels leading to adverse health effects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.

See full target information HFE

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7815 PubMed36535970

2022

Targeting the transcription factor HES1 by L-menthol restores protein phosphatase 6 in keratinocytes in models of psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhikai Wang,Yang Sun,Fangzhou Lou,Jing Bai,Hong Zhou,Xiaojie Cai,Libo Sun,Qianqian Yin,Sibei Tang,Yue Wu,Li Fan,Zhenyao Xu,Hong Wang,Xiaoyu Hu,Honglin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HFE antibody [EPR6750]

Western blot - Anti-HFE antibody [EPR6750] (AB170912)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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