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AB133639

Anti-HFE antibody [EPR6751(2)]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal HFE antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

HLAH, HFE, Hereditary hemochromatosis protein, HLA-H

3 Images
Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)
  • WB

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Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)

All lanes:

Western blot - Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

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Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)
  • WB

AbReview45186****

Western blot - Anti-HFE antibody [EPR6751(2)] (AB133639)

Lanes 1 - 2:

Western blot - Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilution

Lanes 3 - 4:

Rabbit monoclonal anti-myc tag

Lanes 1 and 3:

HepG2 whole cell lysate - untransfected at 25 µg

Lanes 2 and 4:

HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag Myc at 25 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa,60 kDa

true

Exposure time: 5s

This image is courtesy of an Abreview submitted by Chloe Latour

OI-RD Scanning - Anti-HFE antibody [EPR6751(2)] (AB133639)
  • OI-RD Scanning

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OI-RD Scanning - Anti-HFE antibody [EPR6751(2)] (AB133639)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6751(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HFE protein also known as the hemochromatosis protein or HFE 5000 is a critical component in the regulation of iron absorption in the body. Structurally this protein has a known mass of about 50 kDa and is expressed primarily in the liver pancreas and intestines. The HFE belongs to the class I major histocompatibility complex (MHC) proteins. Its main function is to interact with transferrin receptors regulating the amount of iron that's absorbed from the diet into the bloodstream.
Biological function summary

This protein plays a significant role in maintaining iron homeostasis within the body. HFE does not function alone; it forms a complex with transferrin receptors to modulate iron uptake. Its interaction with the transferrin-bound iron helps prevent excess iron build-up by reducing the binding affinity of transferrin for its receptor. Through these interactions HFE balances iron storage and transport ensuring proper iron levels in vital organs.

Pathways

HFE is an integral part of iron metabolism and homeostasis pathways. It closely interacts with proteins like TFR1 (Transferrin Receptor 1) in these pathways. The hepcidin pathway also involves HFE where it indirectly influences the synthesis of hepcidin by the liver. Hepcidin plays an important role in preventing iron overload by binding and degrading the iron export protein ferroportin hence regulating the release of iron from cells adding a layer of control over iron homeostasis.

HFE mutations can lead to hereditary hemochromatosis a condition characterized by excessive iron accumulation in tissues which leads to organ damage. A common mutation is the C282Y mutation which disrupts the protein's normal function. This disorder links to other proteins related to iron metabolism such as hepcidin. Proper functioning of HFE is essential to avoid diseases like hemochromatosis as its impairment results in misregulated iron levels leading to adverse health effects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
See full target information HFE
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 221: PubMed38805014

2024

TET2-STAT3-CXCL5 nexus promotes neutrophil lipid transfer to fuel lung adeno-to-squamous transition.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Xue,Yuting Chen,Sijia Sun,Xinyuan Tong,Yujia Chen,Shijie Tang,Xue Wang,Simin Bi,Yuqin Qiu,Qiqi Zhao,Zhen Qin,Qin Xu,Yingjie Ai,Leilei Chen,Beizhen Zhang,Zhijie Liu,Minbiao Ji,Meidong Lang,Luonan Chen,Guoliang Xu,Liang Hu,Dan Ye,Hongbin Ji

International journal of molecular sciences 23: PubMed35163229

2022

SEC23B Loss-of-Function Suppresses Hepcidin Expression by Impairing Glycosylation Pathway in Human Hepatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Eleni Rosato,Roberta Marra,Vanessa D'Onofrio,Federica Del Giudice,Simone Della Monica,Achille Iolascon,Immacolata Andolfo,Roberta Russo
View all publications
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