AB231968

Anti-HGD antibody

Name: Anti-HGD antibody (ab231968) | Abcam
Brand: Abcam Limited
SKU: AB231968
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HGD antibody. Suitable for WB, IHC-P and reacts with Mouse, Recombinant full length protein - Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Hgd aa 150 to C-terminus.

View Alternative Names

Aku, Hgo, Hgd, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody (AB231968)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody (AB231968)

Paraffin-embedded mouse liver tissue stained for HGD using ab231968 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody (AB231968)

Paraffin-embedded mouse kidney tissue stained for HGD using ab231968 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-HGD antibody (AB231968)

All lanes:

Western blot - Anti-HGD antibody (ab231968) at 3 µg/mL

All lanes:

Recombinant mouse HGD protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-HGD antibody (AB231968)

All lanes:

Western blot - Anti-HGD antibody (ab231968) at 3 µg/mL

Lane 1:

Mouse liver lysate

Lane 2:

Rat liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Hgd aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O09173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231968 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HGD also known as homogentisate oxidase is an important enzyme involved in the catabolism of aromatic amino acids like tyrosine and phenylalanine. This enzyme possesses a molecular mass of roughly 50 kDa. In the human body HGD is expressed predominantly in the liver and kidney tissues. Its primary role is to catalyze the conversion of homogentisic acid into maleylacetoacetic acid a vital step in the breakdown process of these amino acids.

Biological function summary

The role of HGD is essential in maintaining proper amino acid metabolism. It does not directly form part of larger enzymatic complexes but acts as a standalone enzyme that facilitates a specific reaction in the breakdown pathway. This specific reaction aids in preventing the accumulation of intermediary metabolites that could cause cellular toxicity. By regulating the levels of homogentisic acid HGD ensures cellular homeostasis is maintained.

Pathways

The function of HGD fits into several metabolic processes related to amino acid degradation. Most notably it operates within the tyrosine degradation pathway a critical series of reactions required for energy production and detoxification in the body. HGD activity is intricately linked with enzymes like tyrosine aminotransferase which helps initiate the tyrosine breakdown and fumarylacetoacetate hydrolase which serves in the terminal steps of this pathway.

Improper functioning of HGD is directly associated with alkaptonuria a rare hereditary disorder causing the accumulation of homogentisic acid in body tissues. This condition often leads to arthritic complications and kidney stones. The dysfunction of this enzymatic pathway can also indirectly affect vitamin D metabolism highlighting the importance of HGD's efficient operation. In alkaptonuria the insufficient activity of HGD leads to an elevation of homogentisic acid affecting downstream pathways and interacting proteins critical for maintaining skeletal and renal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of homogentisate to maleylacetoacetate.

See full target information Hgd

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 44:417-426 PubMed31173172

2019

Role of TXNIP/NLRP3 in sepsis-induced myocardial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Yang,Wan Xia,Xiaolin Liu,Jian Lin,Aiping Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HGD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody (AB231968)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody (AB231968)

Western blot - Anti-HGD antibody (AB231968)

Western blot - Anti-HGD antibody (AB231968)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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