AB131035

Anti-HGD antibody [EPR7874]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal HGD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

HGO, HGD, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874] (AB131035)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874] (AB131035)

Immunofluorescence analysis of HepG2 cells labelling HGD with ab131035 antibody at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (AB131035)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (AB131035)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HGD with ab131035 antibody at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-HGD antibody [EPR7874] (AB131035)

All lanes:

Western blot - Anti-HGD antibody [EPR7874] (ab131035) at 1/1000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Carrier free

Anti-HGD antibody [EPR7874] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7874

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HGD also known as homogentisate oxidase is an important enzyme involved in the catabolism of aromatic amino acids like tyrosine and phenylalanine. This enzyme possesses a molecular mass of roughly 50 kDa. In the human body HGD is expressed predominantly in the liver and kidney tissues. Its primary role is to catalyze the conversion of homogentisic acid into maleylacetoacetic acid a vital step in the breakdown process of these amino acids.

Biological function summary

The role of HGD is essential in maintaining proper amino acid metabolism. It does not directly form part of larger enzymatic complexes but acts as a standalone enzyme that facilitates a specific reaction in the breakdown pathway. This specific reaction aids in preventing the accumulation of intermediary metabolites that could cause cellular toxicity. By regulating the levels of homogentisic acid HGD ensures cellular homeostasis is maintained.

Pathways

The function of HGD fits into several metabolic processes related to amino acid degradation. Most notably it operates within the tyrosine degradation pathway a critical series of reactions required for energy production and detoxification in the body. HGD activity is intricately linked with enzymes like tyrosine aminotransferase which helps initiate the tyrosine breakdown and fumarylacetoacetate hydrolase which serves in the terminal steps of this pathway.

Improper functioning of HGD is directly associated with alkaptonuria a rare hereditary disorder causing the accumulation of homogentisic acid in body tissues. This condition often leads to arthritic complications and kidney stones. The dysfunction of this enzymatic pathway can also indirectly affect vitamin D metabolism highlighting the importance of HGD's efficient operation. In alkaptonuria the insufficient activity of HGD leads to an elevation of homogentisic acid affecting downstream pathways and interacting proteins critical for maintaining skeletal and renal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of homogentisate to maleylacetoacetate.

See full target information HGD

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 29:100 PubMed38977961

2024

New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Lu,Xiaojian Shi,Qiang Fu,Yaguang Han,Lei Zhu,Zhibin Zhou,Yongchuan Li,Nan Lu

Frontiers in immunology 11:582678 PubMed33072129

2020

Detection of Proximal Tubule Involvement by BK Polyomavirus in Kidney Transplant Recipients With Urinary Sediment Double-Immunostaining.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Huang,Xu-Tao Chen,Shi-Cong Yang,Hui-Fei Yang,Xiao-Tao Hou,Wen-Fang Chen,Jun Li,Rong-Hai Deng,Jin-Quan Luo,Jin-Yuan Wang,Xue Shen,Li-Zhong Chen,Chang-Xi Wang,Jiang Qiu,Gang Huang

Annals of translational medicine 8:235 PubMed32309382

2020

Non-invasive urinary sediment double-immunostaining predicts BK polyomavirus associated-nephropathy in kidney transplant recipients.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Tao Chen,Wen-Fang Chen,Xiao-Tao Hou,Shi-Cong Yang,Hui-Fei Yang,Jun Li,Rong-Hai Deng,Yang Huang,Yelidana Nuertai,Chang-Xi Wang,Jiang Qiu,Gang Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HGD antibody [EPR7874]

Immunocytochemistry/ Immunofluorescence - Anti-HGD antibody [EPR7874] (AB131035)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGD antibody [EPR7874] (AB131035)

Western blot - Anti-HGD antibody [EPR7874] (AB131035)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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