AB24865

Anti-HGF antibody

Name: Anti-HGF antibody (ab24865) | Abcam
Brand: Abcam Limited
SKU: AB24865
Price: 510 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(9 Publications)

Rabbit Polyclonal HGF antibody. Carrier free. Suitable for WB, sELISA and reacts with Human, Recombinant fragment - Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HGF.

View Alternative Names

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF

2 Images

Sandwich ELISA - Anti-HGF antibody (AB24865)

sELISA

Unknown

Sandwich ELISA - Anti-HGF antibody (AB24865)

Standard Curve for HGF (Analyte : ab632) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [EGH2 4C12.1] to HGF (ab10829) at 5ug/ml and Detector Antibody Rabbit polyclonal to HGF (ab24865) at 0.5ug/ml.

Unknown

Western blot - Anti-HGF antibody (AB24865)

All lanes:

Western blot - Anti-HGF antibody (ab24865) at 1 µg/mL

All lanes:

Western blot - Recombinant human HGF protein (<a href='/en-us/products/proteins-peptides/recombinant-human-hgf-protein-ab105061'>ab105061</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 83 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HGF.

P14210

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5μg/ml with <a href='/en-us/products/primary-antibodies/hgf-antibody-egh2-4c121-ab10829'>ab10829</a> as Capture.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.

Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).

See full target information HGF

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 7:e10255 PubMed35079630

2022

Area light source-triggered latent angiogenic molecular mechanisms intensify therapeutic efficacy of adult stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jin Kim,Sung-Won Kim,Gwang-Bum Im,Yeong Hwan Kim,Gun-Jae Jeong,Hye Ran Jeon,Dong-Ik Kim,Haeshin Lee,Sung Young Park,Sung Min Cho,Suk Ho Bhang

Human cell 34:1103-1113 PubMed33860459

2021

Microvesicles derived from human umbilical cord mesenchyme promote M2 macrophage polarization and ameliorate renal fibrosis following partial nephrectomy via hepatocyte growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Du,Guanqun Ju,Jun Zhou,Liang Zhong,Lu Rong,Wenxia Chen,Xiaoli Zhang,Ruijin Zhou,Degang Ding,Tongyu Ji

Cancer control : journal of the Moffitt Cancer Center 26:1073274819875736 PubMed31533461

2019

Aberrant Cyclin E and Hepatocyte Growth Factor Expression, Microvascular Density, and Micro-Lymphatic Vessel Density in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Xie,Yuanyuan Wang,Jingkang He,Zhengzheng Ni,Damin Chai

BMC cancer 19:224 PubMed30866863

2019

8-bromo-7-methoxychrysin suppress stemness of SMMC-7721 cells induced by co-culture of liver cancer stem-like cells with hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wen,Chang Xu,Jie Zhou,Nuo-Min Liu,Ying-Hong Cui,Mei-Fang Quan,Jian-Guo Cao,Kai-Qun Ren

Oncotarget 9:22769-22784 PubMed29854314

2018

Foretinib (GSK1363089) induces p53-dependent apoptosis in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhei Kogata,Tomohito Tanaka,Yoshihiro J Ono,Masami Hayashi,Yoshito Terai,Masahide Ohmichi

Scientific reports 7:15812 PubMed29150644

2017

Therapeutic effects of hepatocyte growth factor-overexpressing dental pulp stem cells on liver cirrhosis in a rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Fang Cao,Shi-Zhu Jin,Liang Sun,Yuan-Bo Zhan,Feng Lin,Ying Li,Ying-Lian Zhou,Xiu-Mei Wang,Li Gao,Bin Zhang

American journal of physiology. Endocrinology and 308:E950-9 PubMed25852006

2015

Estradiol-mediated hepatocyte growth factor is involved in the implantation of endometriotic cells via the mesothelial-to-mesenchymal transition in the peritoneum.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiro J Ono,Masami Hayashi,Akiko Tanabe,Atsushi Hayashi,Masanori Kanemura,Yoshito Terai,Masahide Ohmichi

Stem cell research 13:227-39 PubMed25086214

2014

Signaling through three chemokine receptors triggers the migration of transplanted neural precursor cells in a model of multiple sclerosis.

Applications

IHC-FoFr

Species

Mouse

Mikhal E Cohen,Nina Fainstein,Iris Lavon,Tamir Ben-Hur

The Journal of cell biology 185:743-54 PubMed19433454

2009

Decorin is a novel antagonistic ligand of the Met receptor.

Applications

Species

Human

Silvia Goldoni,Ashley Humphries,Alexander Nyström,Sampurna Sattar,Rick T Owens,David J McQuillan,Keith Ireton,Renato V Iozzo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HGF antibody

Sandwich ELISA - Anti-HGF antibody (AB24865)

Western blot - Anti-HGF antibody (AB24865)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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