JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244931

Anti-HGF antibody [EPR20611-28] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal HGF antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hgf

1 Images
Sandwich ELISA - Anti-HGF antibody [EPR20611-28] - BSA and Azide free (Detector) (AB244931)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-HGF antibody [EPR20611-28] - BSA and Azide free (Detector) (AB244931)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab223862).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20611-28

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab244931 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse HGF. The recommended pair for sandwich ELISA is:
Capture: ab244666, Mouse HGF Capture Antibody (unconjugated)
Detector: ab244931, Mouse HGF Detector Antibody (unconjugated)
The reference range value is 62.5 - 4000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity). Activates MAPK signaling following TMPRSS13 cleavage and activation (By similarity).
See full target information Hgf
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB275901

Human HGF ELISA Kit (Hepatocyte Growth Factor)

Recombinant|SimpleStep

ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

  • 0
  • 0
Assay Kits

AB223862

Mouse HGF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse HGF ELISA Kit (AB223862)

  • 0
  • 0
Primary Antibodies

AB206423

Anti-EGF antibody [EPR19899]

RabMAb|Recombinant

Western blot - Anti-EGF antibody [EPR19899] (AB206423)

  • 0
  • 0
Primary Antibodies

AB51067

Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (AB51067)

  • 5
  • 13
Primary Antibodies

AB246685

Anti-Flt-3 antibody [EPR22163-148] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Flt-3 antibody [EPR22163-148] - BSA and Azide free (Detector) (AB246685)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com