JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313408

Anti-HGF antibody [RP23040069]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Multiclonal HGF antibody. Suitable for ICC/IF, WB, I-ELISA and reacts with Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HGF.

View Alternative Names

HPTA, HGF, Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)

Immunofluorescent analysis of 70% confluent log phase U-87 MG cells fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature labeling HGF with ab313408 at 1 µg/mL concentration followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 at 1/2000 dilution, for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e represents H1299 cells lacking the expression of HGF. Panel f represents control U-87 MG cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HGF Antibody [RP23040069] (AB313408)

Immunofluorescent analysis of HepG2 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling HGF with ab313408 followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of HGF.

Indirect ELISA - Anti-HGF Antibody [RP23040069] (AB313408)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-HGF Antibody [RP23040069] (AB313408)

Indirect ELISA analysis of endogenous HGF in MCF-7 lysate coated onto the plate using ab313408 at various dilutions. A non-linear regression analysis was performed (4 PL) and LOD and LOQ for the antibody were determined.

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)
  • WB

Supplier Data

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)

All lanes:

Western blot - Anti-HGF Antibody [RP23040069] (ab313408) at 1 µg/mL

Lane 1:

U-87 MG cell lysate at 30 µg

Lane 2:

U-87 MG cell lysate treated with Protein Transport Inhibitor cocktail (500X), 1X for 4 Hrs at 30 µg

Lane 3:

H1299 cell lysate at 30 µg

Lane 4:

H1299 cell lysate treated with Protein Transport Inhibitor cocktail (500X), 1X for 4 Hrs at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 83 kDa

true

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)
  • WB

Supplier Data

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)

All lanes:

Western blot - Anti-HGF Antibody [RP23040069] (ab313408) at 1 µg/mL

All lanes:

HepG2 cell lysate

Observed band size: 69 kDa

true

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)
  • WB

Supplier Data

Western blot - Anti-HGF Antibody [RP23040069] (AB313408)

All lanes:

Western blot - Anti-HGF Antibody [RP23040069] (ab313408) at 1 µg/mL

Lane 1:

Non-transfected U-87 MG cell lysate

Lane 2:

Non-targeting scrambled siRNA transfected U-87 MG cell lysate

Lane 3:

Hepatocyte growth factor knockdown U-87 MG cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040069

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, I-ELISA, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HGF.

P14210

style
left-column

Complementary Products

Assay Kits

AB275901

Human HGF ELISA Kit (Hepatocyte Growth Factor)

Recombinant|SimpleStep

ELISA - Human HGF ELISA Kit (Hepatocyte Growth Factor) (AB275901)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB46154

Anti-VEGFA antibody

Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
style
left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The hepatocyte growth factor (HGF) also known as scatter factor (SF) is a protein with a mass of approximately 83 kDa. This protein is mostly expressed in the liver but it can also be found in other organs such as the kidney and the lungs. HGF acts mechanically by binding to the c-Met receptor activating intracellular signaling mechanisms necessary for various physiological processes. Researchers often use HGF ELISA kits to measure its levels in biological samples and study its various roles.
Biological function summary

HGF plays an essential role in cellular growth regeneration and healing processes across different tissues. It is not part of a complex but functions as a single-chain polypeptide that undergoes cleavage into its active form. HGF facilitates cell proliferation and migration; this is especially important during organ regeneration and repair processes. By engaging with its receptor c-Met it induces cell survival and cytoskeletal reorganization making it important for wound healing.

Pathways

HGF is critical in the MAPK and PI3K-Akt signaling pathways which impact processes like cell division and survival. Through these pathways HGF interacts with other proteins such as ERK and Akt contributing to the regulation of cellular growth and metabolism. HGF's role in these pathways highlights its importance in developmental processes and tissue homeostasis.

HGF displays significant connections to cancer and liver cirrhosis. Its overexpression can lead to the development of various carcinomas due to its effect on cell proliferation and migration. In liver cirrhosis the altered HGF-c-Met signaling might contribute to the disease progression and hepatic dysfunction. Additionally HGF can interact with proteins such as transforming growth factor-beta (TGF-β) in these conditions influencing the pathological environment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed : 20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed : 15167892, PubMed : 20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed : 20977675).
See full target information HGF
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com