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AB128926

Anti-HGFA Inhibitor 2 antibody [EPR6283(2)]

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(4 Publications)

Rabbit Recombinant Monoclonal HGFA Inhibitor 2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HAI2, KOP, SPINT2, Kunitz-type protease inhibitor 2, Hepatocyte growth factor activator inhibitor type 2, Placental bikunin, HAI-2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)

ab128926, at 1/250 dilution, staining HGFA Inhibitor 2 in MCF-7 cells by Immunofluorescence.

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)
  • WB

Lab

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926) at 1/1000 dilution

Lane 1:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 34 kDa

false

OI-RD Scanning - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (AB128926)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6283(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HGFA Inhibitor 2 also known as HAI-2 is a serine protease inhibitor involved in regulating the activity of hepatocyte growth factor activator (HGFA). The approximate molecular mass of HAI-2 is 54 kDa. This inhibitor is widely expressed in many tissues but shows especially high expression in the epithelial tissues including the liver kidney and gastrointestinal tract. It often acts near sites of tissue injury or regeneration due to its role in controlling proteolytic activity.
Biological function summary

HGFA Inhibitor 2 is involved in the control of protease-mediated signaling pathways by functioning as a member of a protease inhibitor complex. It plays a role in modulating the activity of proteolytic enzymes which influence tissue remodeling cell growth and angiogenesis. By inhibiting HGFA HAI-2 regulates the conversion of inactive hepatocyte growth factor (HGF) into its active form therefore controlling processes that are important for epithelial cell dynamics.

Pathways

HGFA Inhibitor 2 contributes to the HGF/c-Met signaling pathway and impacts the plasminogen activation system. Through the regulation of HGFA HAI-2 inadvertently influences the activation of c-Met a receptor tyrosine kinase. This interaction is key for numerous processes including cell proliferation motility and morphogenesis. Additionally by affecting the plasminogen activation cascade it helps to modulate extracellular matrix degradation and cellular migration through its association with proteins like uPA and plasminogen.

HGFA Inhibitor 2 relates to conditions such as cancer and chronic kidney disease. It is significant in cancer biology due to its influence on tumor growth and metastasis given its role in the activation of the HGF/c-Met pathway frequently implicated in various cancers. In chronic kidney disease the dysregulation of HAI-2 can disrupt normal protease activity affecting kidney function and potentially contributing to disease progression. Related proteins in these contexts include HGFA and c-Met which are involved in the alteration of cellular behavior in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of HGFAC (PubMed : 9346890). Also inhibits plasmin, and plasma and tissue kallikrein (PubMed : 9115294). Inhibits serine protease activity of TMPRSS13 (PubMed : 20977675, PubMed : 28710277). Inhibits serine protease activity of ST14/matriptase and PRSS8/prostasin in vitro (PubMed : 28710277, PubMed : 30445423).
See full target information SPINT2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 13:1175-1186 PubMed36268559

2022

The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ida Steiro,Esten N Vandsemb,Samah Elsaadi,Kristine Misund,Anne-Marit Sponaas,Magne Børset,Pegah Abdollahi,Tobias S Slørdahl

Cell death & disease 10:435 PubMed31164631

2019

STYK1 promotes tumor growth and metastasis by reducing SPINT2/HAI-2 expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Ma,Dong Liu,Weimiao Li,Shouyin Di,Zhipei Zhang,Jiao Zhang,Liqun Xu,Kai Guo,Yifang Zhu,Jing Han,Xiaofei Li,Xiaolong Yan

Journal of cellular and molecular medicine 23:1562-1571 PubMed30484958

2018

Up-regulation of SPINT2/HAI-2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Marconi Roversi,Nathalia Moreno Cury,Matheus Rodrigues Lopes,Karla Priscila Ferro,João Agostinho Machado-Neto,Marisa Claudia Alvarez,Gabriela Pereira Dos Santos,Renata Giardini Rosa,Ana Leda Longhini,Adriana da Silva Santos Duarte,Fernando Vieira Pericole,Patricia Favaro,José Andres Yunes,Sara Teresinha Olalla Saad

Stem cells and development 23:1109-20 PubMed24410667

2014

Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Marconi Roversi,Matheus Rodrigues Lopes,João Agostinho Machado-Neto,Ana Leda F Longhini,Adriana da Silva Santos Duarte,Mariana Ozello Baratti,Bruna Palodetto,Flávia Adolfo Corrocher,Fernando Vieira Pericole,Paula de Melo Campos,Patricia Favaro,Fabiola Traina,Sara Teresinha Olalla Saad
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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