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AB248225

Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HGFA Inhibitor 2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)

This data was developed using ab128926, the same antibody clone in a different buffer formulation.ab128926, at 1/250 dilution, staining HGFA Inhibitor 2 in MCF-7 cells by Immunofluorescence.

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)
  • WB

Unknown

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)

This data was developed using ab128926, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (ab248225) at 1/1000 dilution

Lane 1:

Fetal kidney tissue lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

Human small intestine lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Lane 5:

PC-3 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 34 kDa

false

OI-RD Scanning - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free (AB248225)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6283(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab248225 is the carrier-free version of ab128926.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HGFA Inhibitor 2 also known as HAI-2 is a serine protease inhibitor involved in regulating the activity of hepatocyte growth factor activator (HGFA). The approximate molecular mass of HAI-2 is 54 kDa. This inhibitor is widely expressed in many tissues but shows especially high expression in the epithelial tissues including the liver kidney and gastrointestinal tract. It often acts near sites of tissue injury or regeneration due to its role in controlling proteolytic activity.
Biological function summary

HGFA Inhibitor 2 is involved in the control of protease-mediated signaling pathways by functioning as a member of a protease inhibitor complex. It plays a role in modulating the activity of proteolytic enzymes which influence tissue remodeling cell growth and angiogenesis. By inhibiting HGFA HAI-2 regulates the conversion of inactive hepatocyte growth factor (HGF) into its active form therefore controlling processes that are important for epithelial cell dynamics.

Pathways

HGFA Inhibitor 2 contributes to the HGF/c-Met signaling pathway and impacts the plasminogen activation system. Through the regulation of HGFA HAI-2 inadvertently influences the activation of c-Met a receptor tyrosine kinase. This interaction is key for numerous processes including cell proliferation motility and morphogenesis. Additionally by affecting the plasminogen activation cascade it helps to modulate extracellular matrix degradation and cellular migration through its association with proteins like uPA and plasminogen.

HGFA Inhibitor 2 relates to conditions such as cancer and chronic kidney disease. It is significant in cancer biology due to its influence on tumor growth and metastasis given its role in the activation of the HGF/c-Met pathway frequently implicated in various cancers. In chronic kidney disease the dysregulation of HAI-2 can disrupt normal protease activity affecting kidney function and potentially contributing to disease progression. Related proteins in these contexts include HGFA and c-Met which are involved in the alteration of cellular behavior in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of HGFAC (PubMed : 9346890). Also inhibits plasmin, and plasma and tissue kallikrein (PubMed : 9115294). Inhibits serine protease activity of TMPRSS13 (PubMed : 20977675, PubMed : 28710277). Inhibits serine protease activity of ST14/matriptase and PRSS8/prostasin in vitro (PubMed : 28710277, PubMed : 30445423).
See full target information SPINT2

Alternative Names

HAI2, KOP, SPINT2, Kunitz-type protease inhibitor 2, Hepatocyte growth factor activator inhibitor type 2, Placental bikunin, HAI-2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20220613 PubMed36694627

2023

circ-IARS depletion inhibits the progression of non-small-cell lung cancer by circ-IARS/miR-1252-5p/HDGF ceRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Yang,Chunping Yang,Ping Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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