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AB150653

Anti-HGS antibody [19-5]

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(1 Publication)

Mouse Monoclonal HGS antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HGS.

View Alternative Names

HRS, HGS, Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110

1 Images
Western blot - Anti-HGS antibody [19-5] (AB150653)
  • WB

Unknown

Western blot - Anti-HGS antibody [19-5] (AB150653)

All lanes:

Western blot - Anti-HGS antibody [19-5] (ab150653) at 1/1000 dilution

Lane 1:

Human brain lysate

Lane 2:

HeLa cell lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

19-5

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HGS.

O14964

Epitope

The epitope for ab150653 is located between the VHS and coiled-coil domain of HGS.

Specificity

The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Lyophilized protein G purified in PBS pH7.4
Storage buffer
pH: 7.2 Constituents: 100% Phosphate Buffer
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HGS protein also known as hepatocyte growth factor-regulated tyrosine kinase substrate plays a significant role in cellular processes. It has a molecular mass of approximately 82 kDa. You will find HGS expressed broadly especially in epithelial tissues where it contributes to regulating various cellular mechanisms. Alternative names for HGS include HRS and HGS products which reflect its involvement in various signaling pathways and functions.
Biological function summary

The HGS acts as a central player in endosomal sorting and targeting processes. It forms a part of the ESCRT-0 complex important for the degradation of surface receptors as it directs ubiquitinated membrane proteins to lysosomes for degradation. HGS interacts with other proteins in the complex such as STAM (Signal transducing adaptor molecule). This role explains its importance in maintaining proper cellular signaling and balance.

Pathways

The HGS protein participates in the endocytosis and ubiquitin-proteasome pathways. HGS plays a critical role in the degradation of epidermal growth factor receptor (EGFR) by assisting its trafficking to lysosomes. This function ties it to cellular communication and signal transduction particularly related to growth signals. Within these pathways HGS closely interacts with proteins such as the hepatocyte growth factor (HGF) and c-Met playing a regulatory role in signal attenuation.

Aberrations in the HGS function link to cancer and neurodegenerative disorders. Dysregulation of the HGS-mediated pathways can lead to abnormal cell growth and cancer progression particularly in epithelial tissues. Additionally research indicates a connection between HGS dysfunction and Alzheimer's disease as it may influence amyloid-beta processing. Interactions with proteins like EGFR and c-Met suggest that alterations in the HGS activity could have significant implications for disease pathogenesis and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
See full target information HGS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 297:101300 PubMed34648765

2021

The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Bodin,Vincent Paillé,Thibauld Oullier,Tony Durand,Philippe Aubert,Catherine Le Berre-Scoul,Philippe Hulin,Michel Neunlist,Moustapha Cissé
View all publications
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Product promise

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