Mouse Monoclonal HGS antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HGS.
Images
![Western blot - Anti-HGS antibody [19-5] (AB150653), expandable thumbnail](https://content.abcam.com/products/images/hgs-antibody-19-5-ab150653--western-blot-img89183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HGS antibody [19-5] (AB150653)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Constituents: 100% Phosphate Buffer- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human HGS. Database link O14964
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Alternative names
HRS, HGS, Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110
Recommended products
Mouse Monoclonal HGS antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HGS.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Constituents: 100% Phosphate Buffer- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human HGS. Database link O14964
- Clone number
- 19-5
- Purification technique
- Affinity purification Protein G
- Specificity
The antibody recognizes an epitope is located between the VHS and coiled-coil domain of the HRS protein.
- Epitope
- The epitope for ab150653 is located between the VHS and coiled-coil domain of HGS.
- Concentration
- Purification notes
Lyophilized protein G purified in PBS pH7.4
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The HGS protein also known as hepatocyte growth factor-regulated tyrosine kinase substrate plays a significant role in cellular processes. It has a molecular mass of approximately 82 kDa. You will find HGS expressed broadly especially in epithelial tissues where it contributes to regulating various cellular mechanisms. Alternative names for HGS include HRS and HGS products which reflect its involvement in various signaling pathways and functions.
- Biological function summary
The HGS acts as a central player in endosomal sorting and targeting processes. It forms a part of the ESCRT-0 complex important for the degradation of surface receptors as it directs ubiquitinated membrane proteins to lysosomes for degradation. HGS interacts with other proteins in the complex such as STAM (Signal transducing adaptor molecule). This role explains its importance in maintaining proper cellular signaling and balance.
- Pathways
The HGS protein participates in the endocytosis and ubiquitin-proteasome pathways. HGS plays a critical role in the degradation of epidermal growth factor receptor (EGFR) by assisting its trafficking to lysosomes. This function ties it to cellular communication and signal transduction particularly related to growth signals. Within these pathways HGS closely interacts with proteins such as the hepatocyte growth factor (HGF) and c-Met playing a regulatory role in signal attenuation.
- Associated diseases and disorders
Aberrations in the HGS function link to cancer and neurodegenerative disorders. Dysregulation of the HGS-mediated pathways can lead to abnormal cell growth and cancer progression particularly in epithelial tissues. Additionally research indicates a connection between HGS dysfunction and Alzheimer's disease as it may influence amyloid-beta processing. Interactions with proteins like EGFR and c-Met suggest that alterations in the HGS activity could have significant implications for disease pathogenesis and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-HGS antibody [19-5] (ab150653), expandable thumbnail](https://content.abcam.com/products/images/hgs-antibody-19-5-ab150653--western-blot-img89183.jpg "Western blot - Anti-HGS antibody [19-5] (ab150653)")
Western blot - Anti-HGS antibody [19-5] (ab150653)
All lanes: Western blot - Anti-HGS antibody [19-5] (ab150653) at 1/1000 dilution
Lane 1: Human brain lysate
Lane 2: HeLa cell lysate
Predicted band size: 86 kDa
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