Anti-HGS antibody

• IP

Supplier Data

Immunoprecipitation - Anti-HGS antibody (AB72053)

Detection of human HGS by western blot of immunoprecipitates.
Samples : Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies : ab72053 used for IP at 6 μg per reaction, lanes 1 and 2, lane 3 is control IgG. For blotting immunoprecipitated HGS, ab72053 was used at 0.4 μg/ml.

All lanes:

Immunoprecipitation - Anti-HGS antibody (ab72053)

Predicted band size: 86 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-HGS antibody (AB72053)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HGS antibody (ab72053) at 0.1 µg/mL

Lane 1:

Renca (Mouse renal adenocarcinoma cell line) whole cell lysate at 15 µg

Lane 2:

CT26.WT (Mouse renal colorectal carcinoma cell line) whole cell lysate at 15 µg

Lane 3:

4T1 (Mouse mammary gland cell line) whole cell lysate at 15 µg

Predicted band size: 86 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-HGS antibody (AB72053)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HGS antibody (ab72053) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 86 kDa

false

Exposure time: 10s