Anti-HGS antibody - C-terminal

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (AB155539)

Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody - C-terminal (AB155539)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-HGS antibody - C-terminal (AB155539)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 86 kDa

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• WB

Supplier Data

Western blot - Anti-HGS antibody - C-terminal (AB155539)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 86 kDa

false

• WB

CiteAb

Western blot - Anti-HGS antibody - C-terminal (AB155539)

HGS western blot using anti-HGS antibody - C-terminal ab155539. Publication image and figure legend from Hammerling, B. C., Najor, R. H., et al., 2017, Nat Commun, PubMed 28134239.

ab155539 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155539 please see the product overview.

ESCRT complexes participate in sequestration of mitochondria.(a) Confocal maximal image projections of Atg5-/- MEFs infected with mCherry-Parkin and treated with DMSO or 25 μM FCCP for the indicated time. Cells were stained with anti-Tim23 or anti-Tom20 (white) to label mitochondria, and for the indicated ESCRT protein (green). Enlarged boxes are single optical slices with arrowheads showing co-localization. Scale bars, 20 μm. (b) Representative WB of isolation of the mitochondrial enriched heavy membrane fraction after 0, 1 or 4 h of 25 μM FCCP treatment in Atg5-/- MEFs overexpressing Parkin. Tim23 is shown as a mitochondrial loading control. (c) Band densitometry of protein levels from b (n=3, *p<0.05 and **p<0.01; ns=not significant versus 0 h FCCP). (d) Quantification of cell death. Atg5-/- MEFs were exposed to DMSO or FCCP (25 μM) for 24 h after siRNA knockdown and then stained with Po-Pro-3. (n=200 screened for cell death in 3 independent experiments, *p<0.05, **p<0.01, ***p<0.001; ns=not significant). (e) Single slice of an electron tomogram showing mitochondria (red) partially sequestered by an early endosome (green) in Atg5-/- MEFs (left). Scale bar, 200 nm. The 3D reconstructed model of mitochondria (red) and endosome (green, right). Movie of the full tomogram of this puncta can be found in Supplementary Movie 2. All values are means±s.e.m. from independent experiments. Statistical significance was calculated using analysis of variance followed by Dunnett's test for multiple comparison. Unprocessed original scans of blots are shown in Supplementary Fig. 11.

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