AB308192

Anti-HGS antibody [HL1730]

Recombinant
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Rabbit Recombinant Monoclonal HGS antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Cat, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human HGS.

View Alternative Names

HRS, HGS, Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs, Protein pp110

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody [HL1730] (AB308192)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody [HL1730] (AB308192)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of HGS protein using ab308192 at 1/250 dilution (green).
Nuclei (blue) is stained with fluoroshield with DAPI.

Supplier Data

Western blot - Anti-HGS antibody [HL1730] (AB308192)

Separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-HGS antibody [HL1730] (ab308192) at 1/1000 dilution

All lanes:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 86.2 kDa

false

Supplier Data

Western blot - Anti-HGS antibody [HL1730] (AB308192)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS antibody [HL1730] (ab308192) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-HGS antibody [HL1730] (ab308192) at 1/1000 dilution

Lane 1:

MDCK cell lysate at 30 µg

Lane 2:

PG-4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 86.2 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1730

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Cat, Dog

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HGS. The exact immunogen used to generate this antibody is proprietary information.

O14964

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HGS protein also known as hepatocyte growth factor-regulated tyrosine kinase substrate plays a significant role in cellular processes. It has a molecular mass of approximately 82 kDa. You will find HGS expressed broadly especially in epithelial tissues where it contributes to regulating various cellular mechanisms. Alternative names for HGS include HRS and HGS products which reflect its involvement in various signaling pathways and functions.

Biological function summary

The HGS acts as a central player in endosomal sorting and targeting processes. It forms a part of the ESCRT-0 complex important for the degradation of surface receptors as it directs ubiquitinated membrane proteins to lysosomes for degradation. HGS interacts with other proteins in the complex such as STAM (Signal transducing adaptor molecule). This role explains its importance in maintaining proper cellular signaling and balance.

Pathways

The HGS protein participates in the endocytosis and ubiquitin-proteasome pathways. HGS plays a critical role in the degradation of epidermal growth factor receptor (EGFR) by assisting its trafficking to lysosomes. This function ties it to cellular communication and signal transduction particularly related to growth signals. Within these pathways HGS closely interacts with proteins such as the hepatocyte growth factor (HGF) and c-Met playing a regulatory role in signal attenuation.

Aberrations in the HGS function link to cancer and neurodegenerative disorders. Dysregulation of the HGS-mediated pathways can lead to abnormal cell growth and cancer progression particularly in epithelial tissues. Additionally research indicates a connection between HGS dysfunction and Alzheimer's disease as it may influence amyloid-beta processing. Interactions with proteins like EGFR and c-Met suggest that alterations in the HGS activity could have significant implications for disease pathogenesis and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

See full target information HGS

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HGS antibody [HL1730]

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody [HL1730] (AB308192)

Western blot - Anti-HGS antibody [HL1730] (AB308192)

Western blot - Anti-HGS antibody [HL1730] (AB308192)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise