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Rabbit Recombinant Monoclonal HHIP antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Hhip antibody [EPR29660-579] (AB324310), expandable thumbnail
  • Western blot - Anti-Hhip antibody [EPR29660-579] (AB324310), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.

Alternative names

HIP, UNQ5825/PRO19644, HHIP, Hedgehog-interacting protein

Rabbit Recombinant Monoclonal HHIP antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29660-579
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310), expandable thumbnail

    Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310)

    Hhip Western blot staining using rabbit Anti-Hhip antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands lower than 79 kDa are unknown.

    To minimize protein degradation, cells (lanes 3) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 81 seconds; Lane 3: 125 seconds.

    All lanes: Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HUVEC transfected with siRNA specifically targeting Hhip whole cell lysate at 20 µg

    Lane 3: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 79 kDa, 36 kDa

  • Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310), expandable thumbnail

    Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310)

    Hhip Western blot staining using rabbit Anti-Hhip antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: CCRF-CE, Jurkat.

    Negative control: HT-29, HepG2 (PMID: 15294024, PMID: 17683069)

    The identity of the bands lower than 79 kDa are unknown.

    To minimize protein degradation, cells (lanes 6) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-6: 37 seconds; Lane 7: 180 seconds.

    All lanes: Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 48 µg

    Lane 2: CCRF-CEM (human peripheral blood T lymphoblast) whole cell lysate at 48 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 48 µg

    Lane 4: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg

    Lane 6: EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 79 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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