Anti-Hhip antibody [EPR29660-579]

• WB

Supplier Data

Western blot - Anti-Hhip antibody [EPR29660-579] (AB324310)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 79 kDa are unknown.

To minimize protein degradation, cells (lanes 3) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 81 seconds; Lane 3 : 125 seconds.

All lanes:

Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HUVEC transfected with siRNA specifically targeting Hhip whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 79 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Hhip antibody [EPR29660-579] (AB324310)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : CCRF-CE, Jurkat.

Negative control : HT-29, HepG2 (PMID : 15294024, PMID : 17683069)

The identity of the bands lower than 79 kDa are unknown.

To minimize protein degradation, cells (lanes 6) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-6 : 37 seconds; Lane 7 : 180 seconds.

All lanes:

Western blot - Anti-Hhip antibody [EPR29660-579] (ab324310) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 48 µg

Lane 2:

CCRF-CEM (human peripheral blood T lymphoblast) whole cell lysate at 48 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 48 µg

Lane 4:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 6:

EA.hy926 (human somatic cell hybrid endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 79 kDa,36 kDa

false