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Rabbit Recombinant Monoclonal HHLA2 antibody. Suitable for IHC-P, WB, IP and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (AB315994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (AB315994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (AB315994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (AB315994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (AB315994), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IFFlow Cyt
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Alternative names

HERV-H LTR-associating protein 2, Human endogenous retrovirus-H long terminal repeat-associating protein 2, HHLA2

Recommended products

Rabbit Recombinant Monoclonal HHLA2 antibody. Suitable for IHC-P, WB, IP and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26395-88
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HHLA2 protein also known as Human endogenous retrovirus-H Long Terminal Repeat-Associating 2 plays a role in the immune response. This protein is part of the B7 family which are known for their involvement in immunomodulation. It has a molecular weight of approximately 30 kDa. HHLA2 is expressed in various tissues including the placenta kidneys intestines and lungs. Additionally HHLA2 is typically upregulated in some cancer tissues which suggests a potential role in tumor immunity.

Biological function summary

HHLA2 acts as a co-inhibitory molecule that modulates T cell activity functioning to suppress T cell proliferation and cytokine production. It engages in the immune checkpoint pathways which are critical for maintaining immune tolerance and preventing over-activation of the immune system. HHLA2 does not act alone but rather interacts with other B7 family proteins in these pathways. It influences CTLA-4 and PD-1 mediated pathways by modulating the immune checkpoints adjusting the immune response based on physiological needs.

Pathways

HHLA2 fits into the immune checkpoint regulation pathways. It is known to modulate the PD-1/PD-L1 pathway which is essential for T cell immune evasion in cancer cells. Through its interactions HHLA2 can impact the tumor microenvironment by influencing immune cell infiltration and activation which is a significant aspect of cancer progression and immune evasion strategies. It is also related to CTLA-4 regulatory pathways which play an important role in maintaining immune homeostasis by negatively regulating T cell activation.

Associated diseases and disorders

HHLA2 associates with cancer particularly certain solid tumors where its expression is increased suggesting its role in immune evasion. This association points to its potential as a therapeutic target for immunotherapy. HHLA2 is connected to proteins like PD-L1 and CTLA-4 which are already known targets in cancer therapy because of their roles in bypassing immune detection by tumors. Additionally its expression is linked to autoimmune diseases where dysregulation of immune checkpoints results in excessive immune cell activity against self-tissues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Immunohistochemical analysis of paraffin-embedded (A) A-204 (human muscle rhabdomyosarcoma cell) cell pellet (B) HeLa (human cervical adenocarcinoma epithelial cell) cell pellet tissue labeling HHLA2 with ab315994 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on A-204 (image A). No staining on Hela (image B). The section was incubated with ab315994 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a human HHLA2 expression vector containing a Myc-His tag. (B) HEK-293T transfected with empty vector containing a Myc-His tag. Tissue labeling HHLA2 with ab315994 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on HEK-293T transfected with a Myc-His-tagged human HHLA2 construct (image A). No staining on HEK-293T transfected with empty plasmid (image B). The section was incubated with ab315994 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling HHLA2 with ab315994 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human spleen (PMID: 32071413). The section was incubated with ab315994 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling HHLA2 with ab315994 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma (PMID: 25549724). The section was incubated with ab315994 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HHLA2 with ab315994 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon (PMID: 25549724). The section was incubated with ab315994 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Immunoprecipitation - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    HHLA2 was immunoprecipitated from 0.35 mg A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate with ab315994 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315994 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate

    Lane 2: ab315994 IP in A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315994 in A-204 whole cell lysate

    All lanes: Immunoprecipitation - Anti-HHLA2 antibody [EPR26395-88] (ab315994) at 1/30 dilution

    All lanes: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-HHLA2 antibody [EPR26395-88] (ab315994), expandable thumbnail

    Western blot - Anti-HHLA2 antibody [EPR26395-88] (ab315994)

    Negative control: HeLa, Jurkat (PMID: 25549724), HepG2.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1: 37 seconds; Lanes 2-7: 10 seconds.

    All lanes: Western blot - Anti-HHLA2 antibody [EPR26395-88] (ab315994) at 1/1000 dilution

    Lane 1: AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HCC827 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: HDLM-2 (human hodgkin lymphoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 7: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 70-80 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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