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AB3836

Anti-hHR23A antibody

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(1 Publication)

Goat Polyclonal hHR23A antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UV excision repair protein RAD23 homolog A aa 100-150.

View Alternative Names

Lysine-specific demethylase RAD23A, UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A

1 Images
Western blot - Anti-hHR23A antibody (AB3836)
  • WB

Unknown

Western blot - Anti-hHR23A antibody (AB3836)

Western blot using ab3836 at 1/500.
Secondary ab : Rabbit anti Goat ab6741 1/5000
Exposure time : 30 seconds

Lane 1 : Hela nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

All lanes:

Western blot - Anti-hHR23A antibody (ab3836)

Predicted band size: 40 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human UV excision repair protein RAD23 homolog A aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P54725

Specificity

This antibody has been shown to detect an approxiametely 60kd band in HeLa and Jurkat lysates. This is comparable in size to the band detected in HeLa in van der Spek et al. 1996. We believe this is good evidence that this antibody is detecting hHR23A.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human homolog of Rad23A or hHR23A is an important player in protein maintenance and degradation pathways. This protein is known for its involvement in DNA repair mechanisms and proteasome function. hHR23A weighs approximately 43 kDa and expresses in many tissues reflecting its diverse roles throughout the body. Scientists also refer to it as RAD23A giving insight into its function by linking with the well-known RAD proteins.
Biological function summary

HHR23A contributes significantly to the protein ubiquitination process an essential cellular mechanism for targeting proteins for degradation. It forms part of a complex with ubiquitin aiding in the recognition and delivery of ubiquitinated proteins to the proteasome for degradation. The protein's ability to bind to both ubiquitin and the proteasome enables its dual function in proteolytic pathways positioning hHR23A as an intermediary that regulates protein turnover and stability.

Pathways

HHR23A engages in the ubiquitin-proteasome pathway and the nucleotide excision repair (NER) pathway. It cooperates with other proteins like Rad4 (XPC in humans) in NER where it functions in DNA damage recognition and repair. In the ubiquitin-proteasome pathway hHR23A interacts with the proteasomal subunit Rpn10 and other ubiquitin-like proteins. These interactions facilitate effective proteolysis of damaged or unnecessary proteins emphasizing its central role in controlling intracellular protein levels.

HHR23A has implications in neurodegenerative diseases and cancer. Impaired ubiquitin-proteasome system function involving hHR23A associates with neurodegenerative diseases such as Alzheimer's where protein aggregates accumulate. hHR23A's interaction with proteins like p53 known for its role in tumor suppression ties this protein to cancer biology highlighting its potential involvement in tumor suppression failure when its pathways are disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation, transcriptional regulation, and viral replication (PubMed : 12643283, PubMed : 14621999, PubMed : 15321727, PubMed : 20614012, PubMed : 32209475, PubMed : 9372924). Specifically demethylates mono-, di- and trimethylated 'Lys-20' of histone H4 (H4K20me1, H4K20me2, H4K20me3, respectively) into unmethylated forms. Activates the transcription of coding genes by demethylating H4K20me1 and the transcription of repetitive elements by demethylating H4K20me3 (PubMed : 32209475). Involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome (PubMed : 12643283, PubMed : 14621999, PubMed : 15321727). Involved in nucleotide excision repair, is considered functionally equivalent to RAD23B in global genome nucleotide excision repair (GG-NER) through its association with XPC. In vitro, the XPC-RAD23A complex demonstrates NER activity (PubMed : 9372924). Can stabilize XPC (By similarity).. (Microbial infection) Involved in -dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
See full target information UV excision repair protein RAD23 homolog A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 596:2746-2767 PubMed35735670

2022

Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.

Applications

Unspecified application

Species

Unspecified reactive species

Alison Sparks,Christopher J Kelly,Mark K Saville
View all publications
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