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AB108592

Anti-hHR23A antibody [EPR4818]

5

(3 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal hHR23A antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A

2 Images
Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] (AB108592)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] (AB108592)

ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by intracellular flow cytometry (shown in red). Rabbit IgG (negative) in green.

Western blot - Anti-hHR23A antibody [EPR4818] (AB108592)
  • WB

Unknown

Western blot - Anti-hHR23A antibody [EPR4818] (AB108592)

All lanes:

Western blot - Anti-hHR23A antibody [EPR4818] (ab108592) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 40 kDa

false

  • Carrier free

    Anti-hHR23A antibody [EPR4818] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4818

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human homolog of Rad23A or hHR23A is an important player in protein maintenance and degradation pathways. This protein is known for its involvement in DNA repair mechanisms and proteasome function. hHR23A weighs approximately 43 kDa and expresses in many tissues reflecting its diverse roles throughout the body. Scientists also refer to it as RAD23A giving insight into its function by linking with the well-known RAD proteins.
Biological function summary

HHR23A contributes significantly to the protein ubiquitination process an essential cellular mechanism for targeting proteins for degradation. It forms part of a complex with ubiquitin aiding in the recognition and delivery of ubiquitinated proteins to the proteasome for degradation. The protein's ability to bind to both ubiquitin and the proteasome enables its dual function in proteolytic pathways positioning hHR23A as an intermediary that regulates protein turnover and stability.

Pathways

HHR23A engages in the ubiquitin-proteasome pathway and the nucleotide excision repair (NER) pathway. It cooperates with other proteins like Rad4 (XPC in humans) in NER where it functions in DNA damage recognition and repair. In the ubiquitin-proteasome pathway hHR23A interacts with the proteasomal subunit Rpn10 and other ubiquitin-like proteins. These interactions facilitate effective proteolysis of damaged or unnecessary proteins emphasizing its central role in controlling intracellular protein levels.

HHR23A has implications in neurodegenerative diseases and cancer. Impaired ubiquitin-proteasome system function involving hHR23A associates with neurodegenerative diseases such as Alzheimer's where protein aggregates accumulate. hHR23A's interaction with proteins like p53 known for its role in tumor suppression ties this protein to cancer biology highlighting its potential involvement in tumor suppression failure when its pathways are disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC : RAD23A dimer has NER activity. Can stabilize XPC.. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.
See full target information UV excision repair protein RAD23 homolog A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5435 PubMed36114200

2022

Electrostatic and steric effects underlie acetylation-induced changes in ubiquitin structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Maria Kienle,Tobias Schneider,Katrin Stuber,Christoph Globisch,Jasmin Jansen,Florian Stengel,Christine Peter,Andreas Marx,Michael Kovermann,Martin Scheffner

Neurotoxicology 68:25-37 PubMed30003905

2018

Comparative proteomics reveals the neurotoxicity mechanism of ER stressors tunicamycin and dithiothreitol.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Ren,Yujuan Wang,Heng Wang,Yingying Wu,Jiayi Li,Jing Tian

Molecular cell 69:866-878.e7 PubMed29499138

2018

The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Keren Baranes-Bachar,Adva Levy-Barda,Judith Oehler,Dylan A Reid,Isabel Soria-Bretones,Ty C Voss,Dudley Chung,Yoon Park,Chao Liu,Jong-Bok Yoon,Wei Li,Graham Dellaire,Tom Misteli,Pablo Huertas,Eli Rothenberg,Kristijan Ramadan,Yael Ziv,Yosef Shiloh
View all publications
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Product promise

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