AB247690

Anti-hHR23A antibody [EPR4818] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal hHR23A antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

UV excision repair protein RAD23 homolog A, HR23A, hHR23A, RAD23A

2 Images

Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] - BSA and Azide free (AB247690)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] - BSA and Azide free (AB247690)

This data was developed using ab108592, the same antibody clone in a different buffer formulation.

ab108592 at 1/10 dilution staining hHR23A in permeabilized Jurkat cells by intracellular flow cytometry (shown in red). Rabbit IgG (negative) in green.

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Western blot - Anti-hHR23A antibody [EPR4818] - BSA and Azide free (AB247690)

This data was developed using ab108592, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-hHR23A antibody [EPR4818] (<a href='/en-us/products/primary-antibodies/hhr23a-antibody-epr4818-ab108592'>ab108592</a>) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 40 kDa

false

Unconjugated

Anti-hHR23A antibody [EPR4818]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4818

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247690 is the carrier-free version of ab108592.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human homolog of Rad23A or hHR23A is an important player in protein maintenance and degradation pathways. This protein is known for its involvement in DNA repair mechanisms and proteasome function. hHR23A weighs approximately 43 kDa and expresses in many tissues reflecting its diverse roles throughout the body. Scientists also refer to it as RAD23A giving insight into its function by linking with the well-known RAD proteins.

Biological function summary

HHR23A contributes significantly to the protein ubiquitination process an essential cellular mechanism for targeting proteins for degradation. It forms part of a complex with ubiquitin aiding in the recognition and delivery of ubiquitinated proteins to the proteasome for degradation. The protein's ability to bind to both ubiquitin and the proteasome enables its dual function in proteolytic pathways positioning hHR23A as an intermediary that regulates protein turnover and stability.

Pathways

HHR23A engages in the ubiquitin-proteasome pathway and the nucleotide excision repair (NER) pathway. It cooperates with other proteins like Rad4 (XPC in humans) in NER where it functions in DNA damage recognition and repair. In the ubiquitin-proteasome pathway hHR23A interacts with the proteasomal subunit Rpn10 and other ubiquitin-like proteins. These interactions facilitate effective proteolysis of damaged or unnecessary proteins emphasizing its central role in controlling intracellular protein levels.

HHR23A has implications in neurodegenerative diseases and cancer. Impaired ubiquitin-proteasome system function involving hHR23A associates with neurodegenerative diseases such as Alzheimer's where protein aggregates accumulate. hHR23A's interaction with proteins like p53 known for its role in tumor suppression ties this protein to cancer biology highlighting its potential involvement in tumor suppression failure when its pathways are disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.. Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC : RAD23A dimer has NER activity. Can stabilize XPC.. (Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.

See full target information UV excision repair protein RAD23 homolog A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hHR23A antibody [EPR4818] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] - BSA and Azide free (AB247690)

Western blot - Anti-hHR23A antibody [EPR4818] - BSA and Azide free (AB247690)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise