AB86781

Anti-hHR23b antibody

Name: Anti-hHR23b antibody (ab86781) | Abcam
Brand: Abcam Limited
SKU: AB86781
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal hHR23b antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RAD23B aa 350 to C-terminus.

View Alternative Names

UV excision repair protein RAD23 homolog B, HR23B, hHR23B, XP-C repair-complementing complex 58 kDa protein, p58, RAD23B

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (AB86781)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (AB86781)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling hHR23b with ab86781 at 1/1000 (0.2 µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-hHR23b antibody (AB86781)

1 mg HeLa whole cell lysate was immunoprecipitated with 10 μg ab86781 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was subjected to Western blotting and labelled with ab86781 at 0.4 μg/ml. Bands were detected by chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-hHR23b antibody (ab86781)

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-hHR23b antibody (AB86781)

All lanes:

Western blot - Anti-hHR23b antibody (ab86781) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 43 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-hHR23b antibody (AB86781)

All lanes:

Western blot - Anti-hHR23b antibody (ab86781) at 0.2 µg/mL

Lane 1:

NIH3T3 whole cell lysate at 50 µg

Lane 2:

TCMK-1 whole cell lysate at 50 µg

Lane 3:

4T1 whole cell lysate at 50 µg

Lane 4:

CT26.WT whole cell lysate at 50 µg

Predicted band size: 43 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-hHR23b antibody (AB86781)

All lanes:

Western blot - Anti-hHR23b antibody (ab86781) at 0.2 µg/mL

All lanes:

C6 (Rat glial tumor cell line) whole cell lysate at 50 µg

Predicted band size: 43 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RAD23B aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human Rad23 homolog B (hHR23b) also known as RAD23B is a protein involved in DNA repair mechanisms. It weighs approximately 43 kDa and is expressed in several tissues including the skin lungs and kidneys. Mechanically hHR23b is important in the process of nucleotide excision repair (NER) where it helps recognize and repair UV-induced DNA damage. hHR23b interacts with other proteins to facilitate the repair process by removing bulky DNA lesions.

Biological function summary

HHR23b plays an important role in maintaining genomic stability. It forms a complex with xeroderma pigmentosum group C (XPC) protein which is instrumental in identifying DNA damage. This complex functions as the initial damage sensor in the NER pathway. By participating in this multiprotein complex hHR23b facilitates the recruitment of other DNA repair factors ultimately ensuring the fidelity of DNA replication.

Pathways

HHR23b is instrumental in NER and proteasome degradation. In the NER pathway it functions alongside the XPC protein to initiate the repair of damaged DNA. Through its interaction with the proteasome system hHR23b is also involved in the ubiquitin-proteasome pathway which regulates protein degradation. This dual involvement in pathways links hHR23b to both DNA repair and the regulation of protein turnover.

HHR23b is associated with cancer and neurodegenerative conditions. The malfunction or altered expression of hHR23b can lead to impaired DNA repair promoting oncogenic transformations and cancer progression specifically through its association with the XPC protein. Furthermore its role in protein degradation connects hHR23b to neurodegenerative diseases like Parkinson's where protein accumulation contributes to disease progression. Understanding these connections helps in devising therapeutic strategies targeting hHR23b and its related pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC : RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC : RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC : RAD23B induces a bend in DNA upon binding. XPC : RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

See full target information RAD23B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 30:4152-4164.e6 PubMed32209475

2020

Histone H4K20 Demethylation by Two hHR23 Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongwen Cao,Yanran Chen,Bin Wu,Xiaoyun Wang,Hongjuan Xue,Lu Yu,Jie Li,Yiqin Wang,Wei Wang,Qing Xu,Hailei Mao,Chao Peng,Gang Han,Charlie Degui Chen

Nature neuroscience 19:668-677 PubMed26998601

2016

C9ORF72 poly(GA) aggregates sequester and impair HR23 and nucleocytoplasmic transport proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Jie Zhang,Tania F Gendron,Jonathan C Grima,Hiroki Sasaguri,Karen Jansen-West,Ya-Fei Xu,Rebecca B Katzman,Jennifer Gass,Melissa E Murray,Mitsuru Shinohara,Wen-Lang Lin,Aliesha Garrett,Jeannette N Stankowski,Lillian Daughrity,Jimei Tong,Emilie A Perkerson,Mei Yue,Jeannie Chew,Monica Castanedes-Casey,Aishe Kurti,Zizhao S Wang,Amanda M Liesinger,Jeremy D Baker,Jie Jiang,Clotilde Lagier-Tourenne,Dieter Edbauer,Don W Cleveland,Rosa Rademakers,Kevin B Boylan,Guojun Bu,Christopher D Link,Chad A Dickey,Jeffrey D Rothstein,Dennis W Dickson,John D Fryer,Leonard Petrucelli

PloS one 8:e68487 PubMed23844208

2013

Calcium-binding capacity of centrin2 is required for linear POC5 assembly but not for nucleotide excision repair.

Applications

Species

Unspecified reactive species

Tiago J Dantas,Owen M Daly,Pauline C Conroy,Martin Tomas,Yifan Wang,Pierce Lalor,Peter Dockery,Elisa Ferrando-May,Ciaran G Morrison

View all publications

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Anti-hHR23b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hHR23b antibody (AB86781)

Immunoprecipitation - Anti-hHR23b antibody (AB86781)

Western blot - Anti-hHR23b antibody (AB86781)

Western blot - Anti-hHR23b antibody (AB86781)

Western blot - Anti-hHR23b antibody (AB86781)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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