AB267355

Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free

Name: Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free (ab267355) | Abcam
Brand: Abcam Limited
SKU: AB267355
Price: 905 USD
Availability: InStock

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Mouse Monoclonal HHV8 antibody. Carrier free. Suitable for IHC-P and reacts with Human gammaherpesvirus 8 samples.

View Alternative Names

HHV 8, Human Herpes Virus 8, KSHV, Kaposi's sarcoma associated herpes virus

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free (AB267355)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free (AB267355)

Formalin-fixed, paraffin-embedded human Kaposi's sarcoma tissue stained for HHV8 using ab267354 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

This image was produced using the same clone but in a different buffer formulation containing PBS, sodium azide and BSA (ab267354).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HHV8/3606

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human gammaherpesvirus 8

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human gammaherpesvirus 8": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incucubation for 30 minutes at room temperature.</p><p>Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.</p>" } } }

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Product details

ab267355 is the carrier-free version of ab267354.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HHV-8 also known as Human Herpesvirus 8 or Kaposi's Sarcoma-associated herpesvirus (KSHV) is a double-stranded DNA virus with a genome mass of approximately 165 kilobase pairs. It mainly expresses in endothelial cells B lymphocytes and epithelial cells. The virus encodes several proteins that mimic host cell proteins influencing cell regulation and survival. One well-documented tool for detection includes HHV-8 immunostain techniques which assist in identifying viral presence in tissues. Important markers such as LN53 are often used in conjunction with HHV-8 IHC (immunohistochemistry) to facilitate accurate identification and study of this virus's expressions.

Biological function summary

HHV-8 disrupts normal cellular processes by expressing proteins like LANA (latency-associated nuclear antigen) that interfere with host cellular mechanisms. It is known to be part of complex interactions within infected cells chiefly affecting the tumor suppressor pathways. These proteins lead to changes that permit the infected cells to escape immune destruction and continue proliferating unchecked. Despite interventions the virus maintains a dormant state in the host which makes complete eradication difficult.

Pathways

HHV-8 influences two major biological pathways—the p53 signaling pathway and the NF-kB signaling pathway. The virus's interference with the p53 protein which plays a critical role in controlling cell cycle arrest and apoptosis is a major aspect of its pathogenic capacity. Additionally HHV-8 can activate the NF-kB pathway which leads to inflammation and survival signaling encouraging oncogenic transformation. This viral manipulation disrupts normal cellular homeostasis and contributes to disease manifestation.

HHV-8 is strongly associated with Kaposi's sarcoma a vascular tumor affecting the skin and other tissues and primary effusion lymphoma. These diseases are connected to the virus through its interactions with host cellular proteins like p53 and the aforementioned pathways. The LANA protein is significant in Kaposi’s sarcoma pathogenesis by binding to p53 and RB protein inhibiting their tumor suppressor functions. Furthermore HHV-8’s ability to remain latent and resist host immune controls complicates treatment and highlights the importance of understanding its role in disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HHV8

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free (AB267355)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise