JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267352

Anti-HHV8 antibody [HHV8/3633R]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal HHV8 antibody. Suitable for IHC-P and reacts with Human gammaherpesvirus 8 samples.

View Alternative Names

HHV 8, Human Herpes Virus 8, KSHV, Kaposi's sarcoma associated herpes virus

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3633R] (AB267352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [HHV8/3633R] (AB267352)

Formalin-fixed, paraffin-embedded human Kaposi's sarcoma tissue stained for HHV8 using ab267352 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HHV8/3633R

Isotype

IgG

Carrier free

No

Reacts with

Human gammaherpesvirus 8

Applications

IHC-P

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human gammaherpesvirus 8": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p><p>Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes.</p>" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HHV-8 also known as Human Herpesvirus 8 or Kaposi's Sarcoma-associated herpesvirus (KSHV) is a double-stranded DNA virus with a genome mass of approximately 165 kilobase pairs. It mainly expresses in endothelial cells B lymphocytes and epithelial cells. The virus encodes several proteins that mimic host cell proteins influencing cell regulation and survival. One well-documented tool for detection includes HHV-8 immunostain techniques which assist in identifying viral presence in tissues. Important markers such as LN53 are often used in conjunction with HHV-8 IHC (immunohistochemistry) to facilitate accurate identification and study of this virus's expressions.
Biological function summary

HHV-8 disrupts normal cellular processes by expressing proteins like LANA (latency-associated nuclear antigen) that interfere with host cellular mechanisms. It is known to be part of complex interactions within infected cells chiefly affecting the tumor suppressor pathways. These proteins lead to changes that permit the infected cells to escape immune destruction and continue proliferating unchecked. Despite interventions the virus maintains a dormant state in the host which makes complete eradication difficult.

Pathways

HHV-8 influences two major biological pathways—the p53 signaling pathway and the NF-kB signaling pathway. The virus's interference with the p53 protein which plays a critical role in controlling cell cycle arrest and apoptosis is a major aspect of its pathogenic capacity. Additionally HHV-8 can activate the NF-kB pathway which leads to inflammation and survival signaling encouraging oncogenic transformation. This viral manipulation disrupts normal cellular homeostasis and contributes to disease manifestation.

HHV-8 is strongly associated with Kaposi's sarcoma a vascular tumor affecting the skin and other tissues and primary effusion lymphoma. These diseases are connected to the virus through its interactions with host cellular proteins like p53 and the aforementioned pathways. The LANA protein is significant in Kaposi’s sarcoma pathogenesis by binding to p53 and RB protein inhibiting their tumor suppressor functions. Furthermore HHV-8’s ability to remain latent and resist host immune controls complicates treatment and highlights the importance of understanding its role in disease development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information HHV8
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB267354

Anti-HHV8 antibody [HHV8/3606]

primary-antibodies

hhv8-antibody-hhv8-3606-ab267354

0

(0 reviews)

Alternative Product
Primary Antibodies

AB267355

Anti-HHV8 antibody [HHV8/3606] - BSA and Azide free

primary-antibodies

hhv8-antibody-hhv8-3606-bsa-and-azide-free-ab267355

0

(0 reviews)

Alternative Product
Primary Antibodies

AB4103

Anti-HHV8 antibody [LN53]

primary-antibodies

hhv8-antibody-ln53-ab4103

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com