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AB250310

Anti-HIAT1 antibody [EPR9642] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HIAT1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HIAT1, MFSD14A, Solute carrier family 71 member 1, Hippocampus abundant transcript 1 protein, Major facilitator superfamily domain-containing 14A, Putative tetracycline transporter-like protein

1 Images
Western blot - Anti-HIAT1 antibody [EPR9642] - BSA and Azide free (AB250310)
  • WB

Supplier Data

Western blot - Anti-HIAT1 antibody [EPR9642] - BSA and Azide free (AB250310)

This data was developed using ab181050, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HIAT1 antibody [EPR9642] (<a href='/en-us/products/primary-antibodies/hiat1-antibody-epr9642-ab181050'>ab181050</a>) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9642

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250310 is the carrier-free version of ab181050.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIAT1 also known as hippocampus abundant transcript 1 protein is a transmembrane protein with a mass of approximately 28 kDa. This protein is located in the cell membrane and shows expression in various tissues but it is more abundant in the brain especially within the hippocampus. Research shows that HIAT1 participates in regulating ion transport across the cell membrane which is important for maintaining cellular homeostasis.
Biological function summary

This protein plays a role in neuronal function and development. It is part of a larger molecular complex that influences synaptic transmission and plasticity. HIAT1 has a significant impact on neuronal signaling by interacting with specific receptors and ion channels. This involvement suggests that the protein has a potential influence on learning and memory processes reflecting its expression pattern in the brain.

Pathways

HIAT1 significantly engages in neural communication pathways that are critical for brain function. It interacts with the glutamatergic signaling pathway which is essential for excitatory synaptic transmission. Connections exist between HIAT1 and other proteins like NMDA receptors contributing to synapse modulation and neuronal excitability. Additionally HIAT1 may influence the calcium signaling pathway to affect cellular responses to stimuli.

Disruptions or alterations in HIAT1 expression have been linked to neurological conditions such as epilepsy and Alzheimer's disease. Abnormal HIAT1 function may affect proteins like amyloid precursor protein (APP) highlighting its potential role in the pathogenesis of Alzheimer's. In epilepsy changes in HIAT1 might contribute to altered neuronal excitability suggesting its involvement in the disease's development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable membrane-bound transporter. May play a role in neuronal nutrient sensing and energy homeostasis. Required for the final stages of spermatogenesis.
See full target information MFSD14A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com