Rabbit Recombinant Monoclonal HIBADH antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-HIBADH antibody [EPR12519(B)] (AB175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--western-blot-img655.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIBADH antibody [EPR12519(B)] (AB175203)")
![Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (AB175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunocytochemistry-immunofluorescence-img54798.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (AB175203)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (AB175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17764.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (AB175203)")
![Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (AB175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--flow-cytometry-intracellular-img53050.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (AB175203)")
![Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (AB175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunoprecipitation-img25777.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (AB175203)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Cow | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Orangutan | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Cow | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Alternative names
HIBADH
Recommended products
Rabbit Recombinant Monoclonal HIBADH antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12519(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIBADH known as 3-hydroxyisobutyrate dehydrogenase plays a role in the catabolic pathway of valine. It catalyzes the oxidation of 3-hydroxyisobutyrate to 3-oxopropanoate. This enzyme weighs approximately 33 kDa. Expression of HIBADH occurs predominantly in the liver kidney and to a lesser extent the heart and skeletal muscle tissues. High expression in these organs suggests its importance in energy metabolism and intermediate metabolite processing.
- Biological function summary
This enzyme facilitates the metabolism of amino acids by converting valine-derived intermediates. HIBADH contributes significantly to energy production particularly during periods of fasting. It is not typically recognized as part of a larger protein complex functioning independently within metabolic pathways. Its role in energy homeostasis indicates its essential part in maintaining cellular function during metabolic stress.
- Pathways
HIBADH is integral to the valine degradation pathway and also impacts the broader branched-chain amino acid catabolism process. These pathways are critical for recycling amino acids for ATP production. HIBADH interacts with other enzymes like acyl-CoA dehydrogenases which further process breakdown products. This interaction highlights HIBADH's connection to a consistent and efficient metabolic network ensuring effective energy yield from valine.
- Associated diseases and disorders
HIBADH has relevance to maple syrup urine disease and its metabolic management. Proper function of HIBADH is essential to avoid accumulation of toxic valine metabolites linked to this condition. Additionally connection to the protein BCKDHA hints at broader implications for amino acid metabolism disorders. BCKDHA part of the branched-chain alpha-ketoacid dehydrogenase complex indicates HIBADH's involvement in a critical intersection of metabolic control and disease prevention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--western-blot-img655.jpg "Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Lanes 1-3: Merged signal (red and green). Green - ab175203 observed at 35 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.ab175203 Anti-HIBADH antibody [EPR12519(B)] was shown to specifically react with HIBADH in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human HIBADH knockout HeLa cell line ab265888 (knockout cell lysate Human HIBADH knockout HeLa cell lysate ab257986) was used. Wild-type and HIBADH knockout samples were subjected to SDS-PAGE. ab175203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HIBADH knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human HIBADH knockout HeLa cell line (Human HIBADH knockout HeLa cell line ab265888)
Lane 3: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 35 kDa, 78 kDa, 80 kDa
Observed band size: 100-125 kDa, 35 kDa, 80 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunocytochemistry-immunofluorescence-img54798.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Immunocytochemistry/ Immunofluorescence - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Immunofluorescent staining of HepG2 cells labeling HIBADH with ab175203 at 1/100 dilution (red). DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17764.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling HIBADH with ab175203 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--flow-cytometry-intracellular-img53050.jpg "Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Flow Cytometry (Intracellular) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling HIBADHwith ab175203at 1/10 dilution (red) compared to a Rabbit IgG (green).
![Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunoprecipitation-img25777.jpg "Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labeling HIBADH with ab175203 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Predicted band size: 35 kDa
![Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--western-blot-img61653.jpg "Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
All lanes: Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203) at 1/1000 dilution
Lane 1: Human fetal liver tissue lysate at 10 µg
Lane 2: Human fetal heart tissue lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: Human fetal kidney tissue lysate at 10 µg
Predicted band size: 35 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54527.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling HIBADH with ab175203 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--western-blot-img61653.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203), expandable thumbnail](https://content.abcam.com/products/images/hibadh-antibody-epr12519b-ab175203--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54527.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBADH antibody [EPR12519(B)] (ab175203)")
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