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AB153826

Anti-HIBCH antibody

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(1 Publication)

Rabbit Polyclonal HIBCH antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HIBCH aa 1-300.

View Alternative Names

3-hydroxyisobutyryl-coenzyme A hydrolase, HIB-CoA hydrolase, HIBYL-CoA-H, HIBCH

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIBCH antibody (AB153826)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIBCH antibody (AB153826)

Immunofluorescence analysis of methanol fixed A431 cells labeling HIBCH with ab153826 at 1/200. Lower panel shows costaining with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBCH antibody (AB153826)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIBCH antibody (AB153826)

Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling HIBCH with ab153826 at 1/500.

Western blot - Anti-HIBCH antibody (AB153826)
  • WB

Unknown

Western blot - Anti-HIBCH antibody (AB153826)

10% SDS PAGE

All lanes:

Western blot - Anti-HIBCH antibody (ab153826) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

Molt4 whole cell lysate at 30 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HIBCH aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q6NVY1

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Complementary Products

Proteins & Peptides

AB124585

Recombinant Human HIBCH protein

SDS-PAGE - Recombinant Human HIBCH protein (AB124585)

  • 0
  • 0
Primary Antibodies

AB109307

Anti-Axin 2 antibody [EPR2005(2)]

RabMAb|Recombinant|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (AB109307)

  • 4
  • 5
Primary Antibodies

AB226396

Anti-PICOT antibody

Immunoprecipitation - Anti-PICOT antibody (AB226396)

  • 0
  • 0
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIBCH or 3-hydroxyisobutyryl-CoA hydrolase is an enzyme involved in the catabolic pathway of valine metabolism. It exhibits a molecular mass of approximately 37 kDa. This enzyme processes 3-hydroxyisobutyryl-CoA into free 3-hydroxyisobutyrate and CoA. HIBCH is expressed in several tissues with high levels found in liver and kidney reflecting its active role in amino acid metabolism.
Biological function summary

The enzyme catalyzes a specific reaction in the branched-chain amino acid metabolic pathway which is an essential process for the degradation and utilization of valine. HIBCH functions independently not forming part of any larger complex. This enzyme is key for maintaining the balance of metabolites derived from valine and is involved in energy production from protein breakdown.

Pathways

HIBCH plays a significant role in the valine degradation pathway where it acts downstream of other enzymes like acyl-CoA dehydrogenase. During this pathway it collaborates with various enzymes to ensure efficient energy extraction from valine. This process links valine metabolism to the larger citric acid cycle where it coordinates with enzymes such as isovaleryl-CoA dehydrogenase.

Mutations or deficiencies in HIBCH correlate with 3-hydroxyisobutyryl-CoA hydrolase deficiency a metabolic disorder that can manifest as developmental delay neurological deficits or acute metabolic crises in severe cases. The enzyme has links to valine metabolism-related disorders where disturbances in its function can lead to elevated levels of neurotoxic intermediates. Its interaction with related enzymes such as branched-chain alpha-keto acid dehydrogenase further accentuates its importance in maintaining metabolic equilibrium.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
See full target information HIBCH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:618 PubMed31409769

2019

Targeting HIBCH to reprogram valine metabolism for the treatment of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Shan,Yuan Gao,Wei Jin,Minmin Fan,Ying Wang,Yanhong Gu,Chenxiao Shan,Lijun Sun,Xin Li,Biao Yu,Qiong Luo,Qiang Xu
View all publications
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Product promise

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