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AB167257

Anti-HIC2 antibody

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(2 Publications)

Mouse Polyclonal HIC2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HIC2.

View Alternative Names

HRG22, KIAA1020, ZBTB30, HIC2, Hypermethylated in cancer 2 protein, Hic-2, HIC1-related gene on chromosome 22 protein, Hic-3, Zinc finger and BTB domain-containing protein 30

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIC2 antibody (AB167257)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIC2 antibody (AB167257)

Immunofluorescence analysis of HepG2 cells labeling HIC2 with ab167257 at 10μg/mL.

Western blot - Anti-HIC2 antibody (AB167257)
  • WB

Unknown

Western blot - Anti-HIC2 antibody (AB167257)

All lanes:

Western blot - Anti-HIC2 antibody (ab167257) at 1 µg/mL

Lane 1:

HIC2 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 66 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HIC2.

Q96JB3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIC2 also known as Hypermethylated in Cancer 2 acts mechanically as a transcriptional repressor. It belongs to the bHLH-PAS family of transcription factors and exhibits a molecular mass of approximately 52 kDa. The protein is ubiquitously expressed with significant expression in heart and skeletal muscle tissues. HIC2 contains a bHLH domain responsible for DNA binding and dimerization facilitating its role in transcriptional repression.
Biological function summary

HIC2 influences gene expression by interacting with other transcription factors. It binds to specific DNA sequences regulating genes involved in cellular differentiation and development. HIC2 often functions within a larger protein complex integrating signals that affect cellular response and proliferation. Due to its regulatory role it plays a part in managing cell cycle and maintaining cellular homeostasis.

Pathways

HIC2 integrates into critical signaling pathways that govern cell growth and differentiation. It participates in the Notch signaling pathway contributing to the regulation of cell fate decisions. HIC2 is also associated with the Hippo pathway which controls organ size and tissue regeneration. These pathways involve interaction with related proteins such as YAP1 in the Hippo pathway and RBPJ in the Notch pathway.

HIC2 displays significant relevance to cancer and congenital heart defects. Altered HIC2 expression or function may contribute to tumorigenesis as it plays a role in cell cycle regulation and apoptosis. Additionally mutations or dysregulation in the HIC2 gene have links to congenital heart defects. Its connections to proteins like TAZ in cancer progression further highlight the importance of HIC2 in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor.
See full target information HIC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:1154-1167 PubMed36650953

2023

Hypermethylation of HIC2 is a potential prognostic biomarker and tumor suppressor of glioma based on bioinformatics analysis and experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Luo,Yifu Liao,Endong Cao,Yong Yang,Kai Tang,Dexiang Zhou,Dong Zhou,Haiping Cai

Oncology reports 36:737-44 PubMed27349208

2016

p12CDK2-AP1 interacts with CD82 to regulate the proliferation and survival of human oral squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Chai,Jun Ju,Shao-Wu Zhang,Zhi-Yuan Shen,Liang Liang,Xiang-Ming Yang,Chao Ma,Qian-Wei Ni,Mo-Yi Sun
View all publications
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Product promise

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