AB175926

Anti-HID1/DMC1 antibody [EPR12901]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal HID1/DMC1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C17orf28, DMC1, HID1, Protein HID1, Down-regulated in multiple cancers 1, HID1 domain-containing protein, Protein hid-1 homolog

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HID1/DMC1 antibody [EPR12901] (AB175926)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HID1/DMC1 antibody [EPR12901] (AB175926)

Immunofluorescent staining of MCF7 cells using labeling HID1/DMC1 with ab175926 at 1/100 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-HID1/DMC1 antibody [EPR12901] (AB175926)

All lanes:

Western blot - Anti-HID1/DMC1 antibody [EPR12901] (ab175926) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

Fetal brain cell lysate at 10 µg

Lane 4:

Human stomach lysate at 10 µg

Lane 5:

SK-BR-3 cell lysate at 10 µg

Lane 6:

HT-29 cell lysate at 10 µg

Predicted band size: 89 kDa

false

Carrier free

Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12901

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HID1 also known as "hiding one" is a protein with a molecular mass of approximately 119 kDa. It plays an important role in intracellular trafficking and signaling. Researchers find HID1 expression mostly in neurons indicating a significant function in the central nervous system. Additionally HID1 is observed to localize within the Golgi apparatus suggesting its involvement in vesicular transport processes.

Biological function summary

HID1 regulates vesicle-mediated transport and neurotransmitter release. It is a part of a multi-protein complex involved in the cellular processes necessary for proper signal transmission between neurons. Within this context HID1 interacts with other endocytic proteins to ensure efficient synaptic vesicle recycling a process essential for maintaining synaptic function and plasticity.

Pathways

HID1's biological function integrates with the synaptic vesicle cycle and intracellular signaling pathways. Specifically it relates to the SNARE complex which is pivotal for vesicle docking and fusion events at synapses. HID1's association with the SNARE complex positions it as a significant factor in the regulation of neurosecretion and synaptic communication.

Research has linked HID1 to neurodegenerative diseases and potentially schizophrenia. Alterations in HID1 expression or function can impact neuronal communication and synaptic integrity contributing to these conditions. Moreover HID1 has shown a connection to the neurodegenerative disorder pathway alongside proteins like SNAP25 suggesting potential interaction or common involvement in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play an important role in the development of cancers in a broad range of tissues.

See full target information HID1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:615 PubMed36460928

2022

The endocytic recycling compartment serves as a viral factory for hepatitis E virus.

Applications

Unspecified application

Species

Unspecified reactive species

Cyrine Bentaleb,Kévin Hervouet,Claire Montpellier,Charline Camuzet,Martin Ferrié,Julien Burlaud-Gaillard,Stéphane Bressanelli,Karoline Metzger,Elisabeth Werkmeister,Maliki Ankavay,Nancy Leon Janampa,Julien Marlet,Julien Roux,Clarence Deffaud,Anne Goffard,Yves Rouillé,Jean Dubuisson,Philippe Roingeard,Cécile-Marie Aliouat-Denis,Laurence Cocquerel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HID1/DMC1 antibody [EPR12901]

Immunocytochemistry/ Immunofluorescence - Anti-HID1/DMC1 antibody [EPR12901] (AB175926)

Western blot - Anti-HID1/DMC1 antibody [EPR12901] (AB175926)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise