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AB249893

Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HID1/DMC1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C17orf28, DMC1, HID1, Protein HID1, Down-regulated in multiple cancers 1, HID1 domain-containing protein, Protein hid-1 homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free (AB249893)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free (AB249893)

This data was developed using ab175926, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of MCF7 cells using labeling HID1/DMC1 with ab175926 at 1/100 dilution (red). DAPI nuclear staining (blue).

Western blot - Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free (AB249893)
  • WB

Supplier Data

Western blot - Anti-HID1/DMC1 antibody [EPR12901] - BSA and Azide free (AB249893)

This data was developed using ab175926, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HID1/DMC1 antibody [EPR12901] (<a href='/en-us/products/primary-antibodies/hid1-dmc1-antibody-epr12901-ab175926'>ab175926</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

Fetal brain cell lysate at 10 µg

Lane 4:

Human stomach lysate at 10 µg

Lane 5:

SK-BR-3 cell lysate at 10 µg

Lane 6:

HT-29 cell lysate at 10 µg

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12901

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249893 is the carrier-free version of ab175926.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HID1 also known as "hiding one" is a protein with a molecular mass of approximately 119 kDa. It plays an important role in intracellular trafficking and signaling. Researchers find HID1 expression mostly in neurons indicating a significant function in the central nervous system. Additionally HID1 is observed to localize within the Golgi apparatus suggesting its involvement in vesicular transport processes.
Biological function summary

HID1 regulates vesicle-mediated transport and neurotransmitter release. It is a part of a multi-protein complex involved in the cellular processes necessary for proper signal transmission between neurons. Within this context HID1 interacts with other endocytic proteins to ensure efficient synaptic vesicle recycling a process essential for maintaining synaptic function and plasticity.

Pathways

HID1's biological function integrates with the synaptic vesicle cycle and intracellular signaling pathways. Specifically it relates to the SNARE complex which is pivotal for vesicle docking and fusion events at synapses. HID1's association with the SNARE complex positions it as a significant factor in the regulation of neurosecretion and synaptic communication.

Research has linked HID1 to neurodegenerative diseases and potentially schizophrenia. Alterations in HID1 expression or function can impact neuronal communication and synaptic integrity contributing to these conditions. Moreover HID1 has shown a connection to the neurodegenerative disorder pathway alongside proteins like SNAP25 suggesting potential interaction or common involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play an important role in the development of cancers in a broad range of tissues.
See full target information HID1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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