AB114977

Anti-HIF-1 alpha antibody

Name: Anti-HIF-1 alpha antibody (ab114977) | Abcam
Brand: Abcam Limited
SKU: AB114977
Price: 500 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(34 Publications)

Anti-HIF-1 alpha antibody (ab114977) is a rabbit polyclonal antibody detecting HIF-1 alpha in IHC-P. Suitable for Human, Mouse.

- Over 20 publications

View Alternative Names

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody (AB114977)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody (AB114977)

ab114977 at 1 : 250 dilution staining HIF-1-alpha in Formalin-fixed, Paraffin-embedded Mouse squamous cell carcinoma tissue by Immunohistochemistry.
Detection : DAB staining.

Supplier Data

Immunocytochemistry - Anti-HIF-1 alpha antibody (AB114977)

Immunocytochemistry analysis of formaldehyde-fixed asynchronous hypoxic HeLa (Human cervix adenocarcinoma epithelial cell line) cells labeling HIF-1 alpha with ab114977 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human HIF1A aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

Q16665

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Reactivity data

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Product details

What is this antibody validated in?
Anti-HIF-1 alpha antibody (ab114977) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human, Mouse samples.

Trusted by the scientific community
Anti-HIF-1 alpha (ab114977) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab114977 was affinity purified using an epitope specific to HIF-1-alpha immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.

Biological function summary

HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.

Pathways

HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.

HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed : 11292861, PubMed : 11566883, PubMed : 15465032, PubMed : 16973622, PubMed : 17610843, PubMed : 18658046, PubMed : 20624928, PubMed : 22009797, PubMed : 30125331, PubMed : 9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed : 11292861, PubMed : 11566883, PubMed : 15465032, PubMed : 16973622, PubMed : 17610843, PubMed : 20624928, PubMed : 22009797, PubMed : 30125331, PubMed : 9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed : 22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed : 16543236, PubMed : 9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed : 10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed : 10202154, PubMed : 10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed : 19528298).. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state (PubMed : 32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed : 32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed : 32697943).

See full target information HIF1A

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Drug delivery 32:2536802 PubMed40754992

2025

Enhancing targeted delivery and efficacy of PEGylated liposomal doxorubicin with liposomal minoxidil: comprehensive in silico, in vitro, and in vivo tumor model studies.

Applications

Unspecified application

Species

Unspecified reactive species

Vahideh Mohammadzadeh,Leila Arabi,Seyedeh Maryam Hosseinikhah,Mohammad Mashreghi,Fatemeh Kalalinia,Neda Mostajeran,Mohammad Reza Zirak,Farzin Hadizadeh,Mojgan Nejabat,Hossein Kamali,Niloufar Rahiman,Mahmoud Reza Jaafari

Communications biology 8:1053 PubMed40665023

2025

Mechanisms and reversibility of nicotine-induced spermatogenesis impairment and DNA methylation changes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajun Cui,Chenglu Wang,Yuxuan Zheng,Yu Zhang,Sisi Luo,Zhuoran Ren,Xueyun Qin,Mo Zhang,Fang Gao,Hefeng Huang,Jing Shu,Guolian Ding

International journal of molecular sciences 26: PubMed40650148

2025

Hypoxic Conditions Promote Cartilage Repair in a Rat Knee Osteochondral Defect Model via Hypoxia-Inducible Factor-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Kei Nakamura,Atsuo Inoue,Yuji Arai,Shuji Nakagawa,Yuta Fujii,Ryota Cha,Keisuke Sugie,Kentaro Hayashi,Tsunao Kishida,Osam Mazda,Kenji Takahashi

Histology and histopathology 40:2047-2058 PubMed40235277

2025

The mechanism of dexmedetomidine regulation of the HIF-1α/FUNDC1 axis in myocardial ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfei Hu,Yidan Huang

PloS one 20:e0319976 PubMed40168275

2025

Intermittent hypoxic stimulation promotes efficient expression of Hypoxia-inducible factor-1α and exerts a chondroprotective effect in an animal osteoarthritis model.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Cha,Shuji Nakagawa,Yuji Arai,Atsuo Inoue,Naoki Okubo,Yuta Fujii,Kenta Kaihara,Kei Nakamura,Tsunao Kishida,Osam Mazda,Kenji Takahashi

Scientific reports 15:4236 PubMed39905044

2025

Untargeted metabolomic and proteomic analysis implicates SIRT2 as a novel therapeutic target for diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijing Zhang,Runze Chang,Heng Wang,Jinshan Chen,Chuanlong Lu,Keyi Fan,Yuhang Zhang,Lizheng Li,Sheng Yan,Honglin Dong

Nature communications 15:9105 PubMed39438477

2024

Epicardial transplantation of antioxidant polyurethane scaffold based human amniotic epithelial stem cell patch for myocardial infarction treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jinying Li,Yuejun Yao,Jiayi Zhou,Zhuoheng Yang,Chen Qiu,Yuwen Lu,Jieqi Xie,Jia Liu,Tuoying Jiang,Yaohui Kou,Zhen Ge,Ping Liang,Cong Qiu,Liyin Shen,Yang Zhu,Changyou Gao,Luyang Yu

Experimental & molecular medicine 56:1574-1590 PubMed38945950

2024

Activation of the osteoblastic HIF-1α pathway partially alleviates the symptoms of STZ-induced type 1 diabetes mellitus via RegIIIγ.

Applications

Unspecified application

Species

Unspecified reactive species

Minglong Qiu,Leilei Chang,Guoqing Tang,Wenkai Ye,Yiming Xu,Nijiati Tulufu,Zhou Dan,Jin Qi,Lianfu Deng,Changwei Li

Cancers 16: PubMed38791872

2024

Partial Hepatectomy Promotes the Development of KRASG12V-Induced Hepatocellular Carcinoma in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Mingkai Zhu,Yan Li,Dong Liu,Zhiyuan Gong

Experimental hematology & oncology 13:11 PubMed38291516

2024

Targeting focal adhesion kinase boosts immune response in KRAS/LKB1 co-mutated lung adenocarcinoma via remodeling the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Qiao,Fei Zhou,Xinyu Liu,Tao Jiang,Haowei Wang,Xuefei Li,Chao Zhao,Lei Cheng,Xiaoxia Chen,Shengxiang Ren,Zaiqi Wang,Caicun Zhou

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HIF-1 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody (AB114977)

Immunocytochemistry - Anti-HIF-1 alpha antibody (AB114977)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

Product promise