Anti-HIF-1 alpha antibody

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p>Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate.</p><p>Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate <a href='/en-us/products/unavailable/hela-dfo-treated-05mm-24h-whole-cell-lysate-ab116322'>ab116322</a>. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended <a href='/en-us/products/tissue-lysates/hela-dfo-treated-05mm-24h-nuclear-lysate-ab180880'>ab180880</a>. The cell fractionation kit can also be purchased separately <a href='/en-us/products/sample-preparation-kits/cell-fractionation-kit-standard-ab109719'>ab109719</a>. Negative Control: Most normal tissues or cells, other than kidney or heart.</p><p>The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below:<br>40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/300 - 1/1000", "WB-species-notes": "<p>Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate.</p><p>Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate <a href='/en-us/products/unavailable/hela-dfo-treated-05mm-24h-whole-cell-lysate-ab116322'>ab116322</a>. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended <a href='/en-us/products/tissue-lysates/hela-dfo-treated-05mm-24h-nuclear-lysate-ab180880'>ab180880</a>. The cell fractionation kit can also be purchased separately <a href='/en-us/products/sample-preparation-kits/cell-fractionation-kit-standard-ab109719'>ab109719</a>. Negative Control: Most normal tissues or cells, other than kidney or heart.</p><p>The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below:<br>40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }