Rabbit Polyclonal HIF-1 alpha antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Human HIF1A aa 350-400 conjugated to Keyhole Limpet Haemocyanin.
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- Journal of orthopaedic surgery and research 18:1692023Diabetes mellitus impairs bone regeneration and biomechanics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeiyu Cai,Yanshi Liu,Kai Liu,Ruomei Zhao,Wenjiao Chen,Aihemaitijiang Yusufu,Yi LiuPubMed 36872328
- Frontiers in immunology 13:9467942022Substratum interactions determine immune response to allogeneic transplants of endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElise C Wilcox,Elazer R EdelmanPubMed 36003373
- British journal of pharmacology 179:4136-41562022Mucin-fused myeloid-derived growth factor (MYDGF164) exhibits a prolonged serum half-life and alleviates fibrosis in chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Du,Ting Wang,Hang Wang,Meijia Yang,Hongping YinPubMed 35393682
- Frontiers in cardiovascular medicine 9:7909212022Melatonin Attenuates Dasatinib-Aggravated Hypoxic Pulmonary Hypertension via Inhibiting Pulmonary Vascular Remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Wang,Jinjin Pan,Jinzhen Han,Miaomiao Gong,Liang Liu,Yunlong Zhang,Ying Liu,Dingyou Wang,Qing Tang,Na Wu,Lin Wang,Jinsong Yan,Hua Li,Yuhui YuanPubMed 35402542
- Scientific reports 12:44622022The possible role of hypoxia in the affected tissue of relapsed clubfoot.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomas Novotny,Adam Eckhardt,Martina Doubkova,Jarmila Knitlova,David Vondrasek,Eliska Vanaskova,Martin Ostadal,Jiri Uhlik,Lucie Bacakova,Jana MusilkovaPubMed 35292718
- Frontiers in pharmacology 12:7889772022Roxadustat (FG-4592) Facilitates Recovery From Renal Damage by Ameliorating Mitochondrial Dysfunction Induced by Folic Acid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Li,Bo Jiang,Yu Zou,Jie Zhang,Yuan-Yuan Fu,Xiao-Yue ZhaiPubMed 35280255
- Frontiers in physiology 13:8044692022Hypoxia During the Consolidation Phase of Distraction Osteogenesis Promotes Bone Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanshi Liu,Jialin Liu,Feiyu Cai,Kai Liu,Xiaoxu Zhang,Aihemaitijiang YusufuPubMed 35283791
- Disease markers 2022:12470072022RIG-I Promotes Cell Viability, Colony Formation, and Glucose Metabolism and Inhibits Cell Apoptosis in Colorectal Cancer by NF-B Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYangyang Liu,Shufang Ye,Yabi Zhu,Luyi Chen,Zizhen ZhangPubMed 35242239
- Scientific reports 12:27012022Inhibition of HIF-1α-AQP4 axis ameliorates brain edema and neurological functional deficits in a rat controlled cortical injury (CCI) model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAo Xiong,Junxia Li,Renping Xiong,Yiming Xia,Xu Jiang,Fuyang Cao,Hong Lu,Jianzhong Xu,Fabo ShanPubMed 35177771
- Phytotherapy research : PTR 36:1736-17472022Cardamonin attenuates cerebral ischemia/reperfusion injury by activating the HIF-1α/VEGFA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongzao Ni,Jinxiao Li,Jinyu Zheng,Botao ZhouPubMed 35142404
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HIF1A aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300 - 1/1000 | Notes Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended ab180880. The cell fractionation kit can also be purchased separately ab109719. Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: |
Species Human | Dilution info 1/300 - 1/1000 | Notes Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended ab180880. The cell fractionation kit can also be purchased separately ab109719. Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/200 | Notes - |
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:16543236, PubMed:9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298). (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state (PubMed:32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed:32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed:32697943).
Alternative names
BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78
Recommended products
Rabbit Polyclonal HIF-1 alpha antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 42 publications. Immunogen corresponding to Synthetic Peptide within Human HIF1A aa 350-400 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HIF1A aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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5 product images
Western blot - Anti-HIF-1 alpha antibody (ab216842)
Cell lysates were first probed with primary antibody (ab216842) at 4°C overnight then incubated with conjugated secondary antibody for 60 min at 37˚C.
All lanes: Western blot - Anti-HIF-1 alpha antibody (ab216842) at 1/1000 dilution
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: U251 cell lysate
Lane 4: HepG2 cell lysate
SecondaryAll lanes: Conjugated secondary antibody at 1/20000 dilution
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody (ab216842)
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with HIF-1 Alpha polyclonal Antibody (1/100, ab216842), 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody (ab216842)
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF-1 Alpha Antibody (ab216842) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Western blot - Anti-HIF-1 alpha antibody (ab216842)
Cell lysate was first probed with primary antibody (ab216842) at 4°C overnight then incubated with conjugated secondary antibody for 90 min at 37˚C.
All lanes: Western blot - Anti-HIF-1 alpha antibody (ab216842) at 1/300 dilution
All lanes: HL60 cell lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution
Predicted band size: 92 kDa
Western blot - Anti-HIF-1 alpha antibody (ab216842)
Cell lysate was first probed with primary antibody (ab216842) at 4°C overnight then incubated with conjugated secondary antibody for 60 min at 37˚C.
All lanes: Western blot - Anti-HIF-1 alpha antibody (ab216842) at 1/1000 dilution
All lanes: U-87MG cell lysate
SecondaryAll lanes: Conjugated secondary antibody at 1/20000 dilution
Predicted band size: 92 kDa
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