AB272040

Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free

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Recombinant
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Rabbit Recombinant Monoclonal HIF-1 alpha antibody. Suitable for IHC-P, ICC, IP, WB, ChIP-seq and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus.

View Alternative Names

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-1 alpha using ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243860).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HIF-1 alpha using ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243860).

Unknown

Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-1 alpha using ab243860 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243860).

Unknown

Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

HIF-1 alpha was immunoprecipitated from 1mg CoCl2 and mock treated HepG2 and HeLa whole cell lysates with ab243860 at 20µg/mL lysate.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243860).

All lanes:

Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] (<a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-bl-124-3f7-ab243860'>ab243860</a>) at 1/1000 dilution

Lane 1:

IP using <a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-bl-124-3f7-ab243860'>ab243860</a> in 200µM CoCl2 treated HepG2 cell lysate

Lane 2:

IP using <a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-bl-124-3f7-ab243860'>ab243860</a> in mock treated HepG2 cell lysate

Lane 3:

IP using <a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-bl-124-3f7-ab243860'>ab243860</a> in 200µM CoCl2 treated HeLa cell lysate

Lane 4:

IP using <a href='/en-us/products/primary-antibodies/hif-1-alpha-antibody-bl-124-3f7-ab243860'>ab243860</a> in mock treated HeLa cell lysate

Lane 5:

IP using Control IgG in 200µM CoCl2 treated HepG2 cell lysate

Predicted band size: 92 kDa

false

Exposure time: 30s

ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

ChIP-seq

Unknown

ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Localization of human HIF1-alpha binding sites in immunoprecipitates from CoCl2 treated Hep-G2 lysate by ChIP-Seq using ab243860This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243860).

Unconjugated

Anti-HIF-1 alpha antibody [BL-124-3F7]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-124-3F7

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P, ICC, ChIP-seq

applications

Immunogen

Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16665

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate.Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate <a href='/en-us/products/unavailable/hela-dfo-treated-05mm-24h-whole-cell-lysate-ab116322'>ab116322</a>. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended <a href='/en-us/products/tissue-lysates/hela-dfo-treated-05mm-24h-nuclear-lysate-ab180880'>ab180880</a>. The cell fractionation kit can also be purchased separately <a href='/en-us/products/sample-preparation-kits/cell-fractionation-kit-standard-ab109719'>ab109719</a>. Negative Control: Most normal tissues or cells, other than kidney or heart.The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "" } } }

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Product details

ab272040 is the BSA-free version of ab243860.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.

Biological function summary

HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.

Pathways

HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.

HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.

See full target information HIF1A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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