Rabbit Recombinant Monoclonal HIF-1 alpha antibody. Suitable for IHC-P, ICC, IP, WB, ChIP-seq and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus.
Images
![ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--chip-sequencing-img9863.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)")
![Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunoprecipitation-img128332.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)")
![Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunocytochemistry-img125669.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82865.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (AB272040)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665
Reactivity data
Select an application| IHC-P | ICC | IP | WB | ChIP-seq | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Antigen retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended ab180880. The cell fractionation kit can also be purchased separately ab109719. Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:16543236, PubMed:9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298). (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state (PubMed:32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed:32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed:32697943).
Alternative names
BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78
Recommended products
Rabbit Recombinant Monoclonal HIF-1 alpha antibody. Suitable for IHC-P, ICC, IP, WB, ChIP-seq and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human HIF1A aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665
- Clone number
- BL-124-3F7
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab272040 is the BSA-free version of Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860.
This product is sold under License from Bethyl Laboratories, Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--chip-sequencing-img9863.jpg "ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)")
ChIP-sequencing - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)
Localization of human HIF1-alpha binding sites in immunoprecipitates from CoCl2 treated Hep-G2 lysate by ChIP-Seq using ab243860This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860).
![Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunoprecipitation-img128332.jpg "Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)")
Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)
HIF-1 alpha was immunoprecipitated from 1mg CoCl2 and mock treated HepG2 and HeLa whole cell lysates with Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 at 20µg/mL lysate.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860).
All lanes: Immunoprecipitation - Anti-HIF-1 alpha antibody [BL-124-3F7] (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860) at 1/1000 dilution
Lane 1: IP using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 in 200µM CoCl2 treated HepG2 cell lysate
Lane 2: IP using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 in mock treated HepG2 cell lysate
Lane 3: IP using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 in 200µM CoCl2 treated HeLa cell lysate
Lane 4: IP using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 in mock treated HeLa cell lysate
Lane 5: IP using Control IgG in 200µM CoCl2 treated HepG2 cell lysate
Predicted band size: 92 kDa
Exposure time: 30s
![Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunocytochemistry-img125669.jpg "Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)")
Immunocytochemistry - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)
Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-1 alpha using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82865.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for HIF-1 alpha using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-bl-124-3f7-bsa-free-ab272040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody [BL-124-3F7] - BSA free (ab272040)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-1 alpha using Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-HIF-1 alpha antibody [BL-124-3F7] ab243860).
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