Name: Anti-HIF-1 alpha antibody - C-terminal
SKU: ab228649
Rating: 5 (2 reviews)

Rabbit Polyclonal HIF-1 alpha antibody. C-terminal. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HIF1A.

Images

ChIP - Anti-HIF-1 alpha antibody - C-terminal (AB228649), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Polyclonal

Immunogen

Recombinant Fragment Protein within Human HIF1A. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665

Consider this alternative

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ChIP	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Tested	Expected
Rat	Predicted	Predicted	Predicted	Predicted	Predicted
Cow	Predicted	Predicted	Predicted	Predicted	Predicted
Dog	Predicted	Predicted	Predicted	Predicted	Predicted
Pig	Predicted	Predicted	Predicted	Predicted	Predicted
Rabbit	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/570	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Cow, Dog, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Cow, Dog, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500 - 1/10000	Notes Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended ab180880. The cell fractionation kit can also be purchased separately ab109719. Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta
Species Human	Dilution info 1/500 - 1/10000	Notes Ensure cell lysis occurs quickly if removed from hypoxia. Ultrasonic lysis is recommended to enrich more nuclear lysate. Positive Control: Hypoxic samples such as HeLa-DFO treated whole cell lysate ab116322. For a stronger signal, HeLa-DFO treated nuclear extracts are recommended ab180880. The cell fractionation kit can also be purchased separately ab109719. Negative Control: Most normal tissues or cells, other than kidney or heart. The observed band size of HIF-1 alpha is not exactly as predicted due to the different forms of HIF-1 alpha below: 40-80 kDa - degraded; 10-130 kDa - post translationally modified; ~200 kDa - heterodimer with HIF-1 beta

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Cow, Dog, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100 - 1/1000	Notes -
Species Human	Dilution info 1/100 - 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Cow, Dog, Pig	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Rat, Rabbit, Cow, Dog, Pig	Dilution info -	Notes -

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

See full target information HIF1A

Function

Functions as a master transcriptional regulator of the adaptive response to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:18658046, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia (PubMed:11292861, PubMed:11566883, PubMed:15465032, PubMed:16973622, PubMed:17610843, PubMed:20624928, PubMed:22009797, PubMed:30125331, PubMed:9887100). Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease (PubMed:22009797). Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300 (PubMed:16543236, PubMed:9887100). Activity is enhanced by interaction with NCOA1 and/or NCOA2 (PubMed:10594042). Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP (PubMed:10202154, PubMed:10594042). Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (PubMed:19528298). (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent pro-inflammatory state (PubMed:32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed:32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed:32697943).

Alternative names

BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Polyclonal
Immunogen: Recombinant Fragment Protein within Human HIF1A. The exact immunogen used to generate this antibody is proprietary information. Database link Q16665
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.

Biological function summary

HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.

Pathways

HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.

Associated diseases and disorders

HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

ChIP - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either normal rabbit IgG or ab228649. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 500 µM CoCl2 for 24 hours, whole cell extract at 30 µg
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF-1 alpha (green) using ab228649 at 1/500 dilution in ICC/IF.
Counterstain: Phalloidin, a cytoskeleton marker, at 1/100 dilution (red).
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/500 dilution
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 2: NIH/3T3 (mouse embryo fibroblast cell line) treated with 500 µM CoCl2 for 24 hours, whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 92 kDa
Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
HIF-1 alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 μM CoCl2 for 24 hours, whole cell extract using 5 μg of ab228649. Western blot was performed from the immunoprecipitate using ab228649.
Lane 1: Control IgG IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.
Lane 2: ab228649 IP in HepG2 treated with 500 μM CoCl2 for 24 hours, whole cell extracts.
All lanes: Immunoprecipitation - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
Predicted band size: 92 kDa
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/5000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Flag-tagged HIF-1 alpha, whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 92 kDa
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution
Lane 1: Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) treated with 1% O2 for 24 hours, whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
4% paraformaldehyde-fixed, treated and mock-treated NIH/3T3 (mouse embryo fibroblast cell line) cells stained for HIF-1 alpha (green) using ab228649 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/5000 dilution
Lane 1: Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) treated with 200 µM CoCl2 for 24 hours, whole cell lysate at 30 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) treated with 500 µM CoCl2 for 24 hours, whole cell lysate at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 60 kDa, 81 kDa, 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
Paraffin-embedded human kidney cancer tissue stained for HIF-1 alpha using ab228649 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-HIF-1 alpha antibody - C-terminal (ab228649) at 1/1000 dilution
Lane 1: Non-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg
Lanes 2, 3 and 5: HIF-1 alpha shRNA-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg
Lane 4: HIF-1 alpha shRNA-transfected, untreated MCF7 (human breast adenocarcinoma cell line) whole cell extracts at 30 µg
Lane 6: Non-transfected MCF7 (human breast adenocarcinoma cell line) treated with 1% O2, whole cell extract at 30 µg
Lanes 10, 7, 8 and 9: HIF-1 alpha shRNA-transfected MCF7 (human breast adenocarcinoma cell line) treated with 1% O2, whole cell extract at 30 µg
Secondary
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody - C-terminal (ab228649)
4% paraformaldehyde-fixed untreated (left panel) and 500 μM CoCl₂ treated (right panel) HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HIF-1 alpha (green) using ab228649 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Phalloidin, a cytoskeleton marker, at 1/100 dilution (red).