Knockout Tested Rabbit Recombinant Multiclonal HIF-1 alpha antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432584.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045)")
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432583.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045)")
![Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--flow-cytometry-intracellular-img432628.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045)")
![Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--flow-cytometry-intracellular-img432627.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045)")
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432568.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (AB317045)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | WB | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Expected | Expected | Tested | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
The protein expressed by the gene HIF1A functions as a master transcriptional regulator of the adaptive response to hypoxia, activating the transcription of over 40 genes under hypoxic conditions, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and others. These genes' protein products enhance oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A is crucial for embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. Its activation requires transcriptional coactivators like CREBBP and EP300, with activity enhanced by interactions with NCOA1 and/or NCOA2. Interaction with redox regulatory protein APEX1 activates CTAD and enhances activation by NCOA1 and CREBBP. Additionally, HIF1A is involved in axonal distribution and mitochondrial transport in neurons during hypoxia. In the context of microbial infection, specifically human coronavirus SARS-CoV-2, HIF1A is necessary for glycolysis induction in monocytes, leading to a proinflammatory state, inducing expression of ACE2, cytokines, and promoting virus replication and monocyte inflammatory response. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
BHLHE78, MOP1, PASD8, HIF1A, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, bHLHe78
Recommended products
Knockout Tested Rabbit Recombinant Multiclonal HIF-1 alpha antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1116
- Purification technique
- Affinity purification Protein A
- Specificity
HIF-1 alpha expression is absent in most normal tissues (PMID: 12128120, 24835245, 11689469, 20217131).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317045 is the carrier-free version of Anti-HIF-1 alpha antibody [RM1116] ab317044.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
- Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
- Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
- Associated diseases and disorders
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432584.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HIF-1 alpha with Anti-HIF-1 alpha antibody [RM1116] ab317044 at 1/200 (2.53 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing increased nuclear staining in HeLa cells treated with 1mM DFO for 24 hours (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432583.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HIF1A KO HCT116(HIF1A knockout human colorectal carcinoma epithelial cell),Human HIF1A (HIF-1 alpha) knockout HCT116 cell line ab255394 cells labelling HIF-1 alpha with Anti-HIF-1 alpha antibody [RM1116] ab317044 at 1/200 (2.53 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing negative staining in both HIF1A knockout HCT 116 cells and wildtype HCT 116 cells, the nuclear signal was increased in wildtype HCT 116 cells treated with 0.5 mM DFO for 24 hours (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--flow-cytometry-intracellular-img432628.jpg "Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized bEnd.3 (mouse brain endothelial cell) treated with 0.5mM DFO for 24 hours(Red) / Untreated bEnd.3 (Red Dotted) cells labelling HIF-1 alpha with Anti-HIF-1 alpha antibody [RM1116] ab317044 at 1/5000 dilution (0.01 ug)/Red and Dotted Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--flow-cytometry-intracellular-img432627.jpg "Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Flow Cytometry (Intracellular) - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT116 (human colon epithelial) treated with 0.5mM DFO for 24 hours(Red) / Untreated HCT116 (Red Dotted) cells labelling HIF-1 alpha with Anti-HIF-1 alpha antibody [RM1116] ab317044 at 1/5000 dilution (0.01 ug)/Red and Dotted Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432568.jpg "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Lysates/proteins at 20 MSD1483 per lane.
In Western blot, Anti-HIF-1 alpha antibody [RM1116] ab317044 was shown to bind specifically to HIF-1 alpha. A band was observed at 110 kDa in wild-type HCT 116 cell lysates with whereas no signal observed at this size in HIF-1 alpha knockout cell line Human HIF1A (HIF-1 alpha) knockout HCT116 cell line ab255394.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HIF-1 alpha antibody [RM1116] (Anti-HIF-1 alpha antibody [RM1116] ab317044) at 1/1000 dilution
Lane 1: Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: HCT116 treated with 0.5 mM DFO for 24 hour whole cell lysate at 20 µg with NFDM/TBST
Lane 3: Untreated HIF-1 alpha knockout HCT116 whole cell lysate at 20 µg with NFDM/TBST
Lane 4: HIF-1 alpha knockout HCT116 treated with 0.5 mM DFO for 24 hour whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 110 kDa, 36 kDa
Exposure time: 26s
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432570.jpg "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
The bands beneath the target band (110 kDa) are likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HIF-1 alpha antibody [RM1116] (Anti-HIF-1 alpha antibody [RM1116] ab317044) at 1/1000 dilution
Lane 1: Untreated bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: bEnd.3 treated with 0.5mM DFO for 24 hours whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 110 kDa, 36 kDa
Exposure time: 15s
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432569.jpg "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID:23076220)
The band beneath the target band is likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HIF-1 alpha antibody [RM1116] (Anti-HIF-1 alpha antibody [RM1116] ab317044) at 1/1000 dilution
Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: HeLa treated with 0.5mM CoCl2 for 6 hours whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 110 kDa, 36 kDa
Exposure time: 6s
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432585.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)
This data was developed using Anti-HIF-1 alpha antibody [RM1116] ab317044, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling HIF-1 alpha with Anti-HIF-1 alpha antibody [RM1116] ab317044 at 1/200 (2.53 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing increased nuclear staining in bEnd.3 cells treated with 0.5 mM DFO for 24 hours (shown in green). The counterstain was observed in red. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432570.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
![Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--western-blot-img432569.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
![Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045), expandable thumbnail](https://content.abcam.com/products/images/hif-1-alpha-antibody-rm1116-bsa-and-azide-free-ab317045--immunocytochemistry-immunofluorescence-img432585.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [RM1116] - BSA and Azide free (ab317045)")
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