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Rabbit Recombinant Monoclonal HIF-2-alpha antibody. Suitable for IHC-P, ICC, IP, WB, ICC/IF, ChIP-seq and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EPAS1 aa 400-450.
Alternative names=Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha, PASD2, HIF2A, BHLHE73, MOP2, EPAS1
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human EPAS1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link: Q99814
Reactivity data
Select an application| IHC-P | ICC | IP | WB | ICC/IF | ChIP-seq | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Tested |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Antigen retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker and overnight incubations are recommended. Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 5-20μl/mg lysate. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10.00000-40.00000 µL for 30 µg chromatin | Notes - |
Recommended products
Rabbit Recombinant Monoclonal HIF-2-alpha antibody. Suitable for IHC-P, ICC, IP, WB, ICC/IF, ChIP-seq and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EPAS1 aa 400-450.
Alternative names=Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha, PASD2, HIF2A, BHLHE73, MOP2, EPAS1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human EPAS1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link: Q99814
- Clone number
BL-95-1A2
- Concentration
- Purification notes
Recombinant antibody was purified from cell culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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