Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

• ICC

Unknown

Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

• IP

Unknown

Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 μL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution.

Lane 1 : ab243861 IP in HepG2 cell lysate treated with 200 μM CoCl2.

Lane 2 : ab243861 IP in HepG2 whole cell lysate Mock treated.

Lane 3 : Contol IgG in HepG2 cell lysate treated with 200 μM CoCl2.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

All lanes:

Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] (<a href='/en-us/products/primary-antibodies/hif-2-alpha-antibody-bl-95-1a2-ab243861'>ab243861</a>)

Predicted band size: 96 kDa

false

• ChIP-seq

Unknown

ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

Localization of human HIF2-alpha binding sites in immunoprecipitates from subcutaneous tumor 786-O cells by ChIP-Seq using ab243861.This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

• WB

Supplier Data

Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (AB272041)

Chemiluminescence with exposure time of 3 minutes.

All lanes:

Western blot - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041) at 1/1000 dilution

Lane 1:

HepG2 treated with 200 µM CoCl2 at 15 µg

Lane 2:

HepG2 mock treated at 15 µg

Lane 3:

HEK-293T treated with 200 µM CoCl2 at 15 µg

Lane 4:

HEK-293T mock treated at 15 µg

Lane 5:

786-O cells treated with 200 µM CoCl2 at 15 µg

Lane 6:

786-O cells mock treated at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 96 kDa

false