Rabbit Recombinant Monoclonal HIF-2-alpha antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![ELISA - Anti-HIF-2-alpha antibody [EPR19656-33] - BSA and Azide free (Detector) (AB244956), expandable thumbnail](https://content.abcam.com/products/images/hif-2-alpha-antibody-epr19656-33-bsa-and-azide-free-detector-ab244956--elisa-img35853.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-HIF-2-alpha antibody [EPR19656-33] - BSA and Azide free (Detector) (AB244956)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).
Alternative names
Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha, PASD2, HIF2A, BHLHE73, MOP2, EPAS1
Recommended products
Rabbit Recombinant Monoclonal HIF-2-alpha antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, HIF-1-alpha-like factor, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, bHLHe73, HLF, HIF-2-alpha, HIF2-alpha, PASD2, HIF2A, BHLHE73, MOP2, EPAS1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19656-33
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab244956 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human HIF-2-alpha. The recommended pair for sandwich ELISA is:
Capture: Anti-HIF-2-alpha antibody [EPR19656-21] - BSA and Azide free (Capture) ab244690, Human HIF-2-alpha Capture Antibody (unconjugated)
Detector: ab244956, Human HIF-2-alpha Detector Antibody (unconjugated)
The reference range value is 31.3 - 2000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
HIF-2-alpha also known as hypoxia-inducible factor 2-alpha or EPAS1 plays a significant role in cellular response to oxygen levels. Mechanically HIF-2-alpha functions as a transcription factor that activates certain genes when oxygen is low. The protein typically weighs around 118 kDa. It is expressed in various tissues but particularly in endothelial cells and some neuronal tissues. These sites highlight its importance in organs requiring tight regulation of oxygen.
- Biological function summary
HIF-2-alpha regulates the expression of genes involved in energy metabolism and angiogenesis. The protein forms a complex by dimerizing with the HIF-1-beta subunit which is necessary for transcriptional activity. Through this complex formation and activity it influences processes such as erythropoiesis and regulates factors like vascular endothelial growth factor (VEGF). Therefore HIF-2-alpha contributes to the adaptation of cells and tissues under hypoxic conditions.
- Pathways
HIF-2-alpha engages in the hypoxia signaling pathway playing an essential part by modulating gene expression in response to low oxygen availability. This role impacts other proteins such as HIF-1-alpha sharing overlapping functions but with distinct target genes. Additionally HIF-2-alpha is involved in the mTOR pathway which influences cell growth and metabolism through its interaction with the nutrient-sensing regulatory pathway.
- Associated diseases and disorders
Researchers have linked HIF-2-alpha with conditions like renal cell carcinoma and pulmonary hypertension. The protein's dysregulation can lead to altered expression of angiogenic factors promoting tumor growth and survival. In particular its connection with VEGF exemplifies its role in these pathologies by facilitating aberrant blood vessel formation. Such interactions further highlight the potential of HIF-2-alpha as a therapeutic target in disease modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![ELISA - Anti-HIF-2-alpha antibody [EPR19656-33] - BSA and Azide free (Detector) (ab244956), expandable thumbnail](https://content.abcam.com/products/images/hif-2-alpha-antibody-epr19656-33-bsa-and-azide-free-detector-ab244956--elisa-img35853.jpg "ELISA - Anti-HIF-2-alpha antibody [EPR19656-33] - BSA and Azide free (Detector) (ab244956)")
ELISA - Anti-HIF-2-alpha antibody [EPR19656-33] - BSA and Azide free (Detector) (ab244956)
HELA cells were untreated or treated with 250 µM DFO for 24 hrs, then collected and extracted according to sample preparation protocol (see section 11.1). The concentrations of HIF-2-alpha were measured in duplicate and interpolated from the HIF-2-alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HIF-2-alpha concentration was determined to be 520 pg/mL in DFO treated HELA cell extract and undetectable in untreated HELA cell extract.
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