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AB289550

Anti-HIF1 beta antibody [29/HIF-1b]

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Mouse Recombinant Monoclonal HIF1 beta antibody. Suitable for ICC/IF, WB and reacts with Mouse, Human samples.

View Alternative Names

BHLHE2, ARNT, Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HIF1 beta with ab289550 at 1/50 (21.24 μg/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line ab179513. Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab289550 at 1/50 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

Negative control 2 : ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling HIF1 beta with ab289550 at 1/50 (21.24 μg/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cell line ab179513. Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab289550 at 1/50 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

Negative control 2 : ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Western blot - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)
  • WB

Supplier Data

Western blot - Anti-HIF1 beta antibody [29/HIF-1b] (AB289550)

Blocking and diluting buffer : 5% NFDM/TBST.

Lysates should be made freshly and used in WB immediately to minimize protein degradation.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-HIF1 beta antibody [29/HIF-1b] (ab289550) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 95 kDa

false

  • Carrier free

    Anti-HIF1 beta antibody [29/HIF-1b] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

29/HIF-1b

Isotype

IgG1

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.
Biological function summary

HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.

Pathways

The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.

HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed : 34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).
See full target information ARNT
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Product promise

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For full details, please see our Terms & Conditions

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