AB232964

Anti-HIF1 beta antibody

Name: Anti-HIF1 beta antibody (ab232964) | Abcam
Brand: Abcam Limited
SKU: AB232964
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HIF1 beta antibody. Suitable for WB, ICC/IF and reacts with Rat, Pig, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARNT aa 600 to C-terminus.

View Alternative Names

BHLHE2, ARNT, Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody (AB232964)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody (AB232964)

HepG2 (human liver hepatocellular carcinoma cell line) cells labelling HIF1 beta (green) using ab232964 at 20 μg/ml in ICC/IF, followed by FITC staining.

Supplier Data

Western blot - Anti-HIF1 beta antibody (AB232964)

All lanes:

Western blot - Anti-HIF1 beta antibody (ab232964) at 2 µg/mL

Lane 1:

Human serum

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 3:

Pig kidney lysate

Lane 4:

Rat placenta lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 87 kDa

false

Supplier Data

Western blot - Anti-HIF1 beta antibody (AB232964)

All lanes:

Western blot - Anti-HIF1 beta antibody (ab232964) at 2 µg/mL

All lanes:

Recombinant human HIF1 beta protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ARNT aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P27540

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-20 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab232964 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.

Biological function summary

HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.

Pathways

The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.

HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed : 34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT : AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed : 28396409).

See full target information ARNT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012305 PubMed38905309

2024

PoRVA G9P[23] and G5P[7] infections differentially promote PEDV replication by reprogramming glutamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Haixin Liu,Haolun Tian,Pengcheng Hao,Huimin Du,Kun Wang,Yudong Qiu,Xiangrui Yin,Nana Wu,Qian Du,Dewen Tong,Yong Huang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HIF1 beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody (AB232964)

Western blot - Anti-HIF1 beta antibody (AB232964)

Western blot - Anti-HIF1 beta antibody (AB232964)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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