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Rabbit Recombinant Monoclonal HIF1 beta antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-HIF1 beta antibody [EPR17516] (AB184711), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (AB184711), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (AB184711), expandable thumbnail
  • Western blot - Anti-HIF1 beta antibody [EPR17516] (AB184711), expandable thumbnail
  • Western blot - Anti-HIF1 beta antibody [EPR17516] (AB184711), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Required for activity of the AHR. Upon ligand binding, AHR translocates into the nucleus, where it heterodimerizes with ARNT and induces transcription by binding to xenobiotic response elements (XRE). Not required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding (PubMed:34521881). The complex initiates transcription of genes involved in the regulation of a variety of biological processes, including angiogenesis, hematopoiesis, drug and lipid metabolism, cell motility and immune modulation (Probable). The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity). The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).

Alternative names

BHLHE2, ARNT, Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, Hypoxia-inducible factor 1-beta, bHLHe2, HIF-1-beta, HIF1-beta

Recommended products

Rabbit Recombinant Monoclonal HIF1 beta antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17516
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.

Biological function summary

HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.

Pathways

The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.

Associated diseases and disorders

HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Lanes 1 - 4: Merged signal (red and green). Green - ab184711 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab184711 was shown to react with ARNT in HAP1 wild-type cells in Western blot. Loss of signal was observed when ARNT knockout sample was used. HAP1 wild-type and ARNT knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab184711 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: ARNT knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Lane 4: HepG2 whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 87 kDa

    Observed band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling HIF1 beta with ab184711 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear staining on epithelial cells of Human uterus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HIF1 beta with ab184711 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear staining on cancer cells of hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution

    All lanes: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa, 87 kDa

    Observed band size: 54 kDa, 87 kDa

    Exposure time: 15s

  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/1000 dilution

    All lanes: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 150 kDa, 27 kDa, 87 kDa

    Observed band size: 130 kDa, 180 kDa, 30 kDa, 87 kDa

    Exposure time: 8s

  • Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711), expandable thumbnail

    Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HIF1 beta antibody [EPR17516] (ab184711) at 1/2000 dilution

    Lane 1: Human pancreas lysate at 10 µg

    Lane 2: Human colon lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 87 kDa

    Observed band size: 87 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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