AB176464

Anti-HIF3 alpha/IPAS antibody

Name: Anti-HIF3 alpha/IPAS antibody (ab176464) | Abcam
Brand: Abcam Limited
SKU: AB176464
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal HIF3 alpha/IPAS antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HIF3A aa 450-550.

View Alternative Names

BHLHE17, MOP7, PASD7, HIF3A, Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF3-alpha-1, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, bHLHe17, IPAS

1 Images

Supplier Data

Western blot - Anti-HIF3 alpha/IPAS antibody (AB176464)

All lanes:

Western blot - Anti-HIF3 alpha/IPAS antibody (ab176464) at 0.1 µg/mL

All lanes:

Rat brain tissue lysate

Predicted band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human HIF3A aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2N7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIF3 alpha also known as IPAS stands as a unique member of the hypoxia-inducible factor (HIF) family involved in oxygen regulation. HIF3 alpha is a transcription factor made of approximately 66 kDa protein mass which plays a role in controlling gene expression in response to low oxygen conditions. It gets expressed in various tissues including the brain lungs and kidneys. Unlike HIF1 alpha and HIF2 alpha HIF3 alpha can act as negative regulator of HIF-mediated gene activation by forming complexes with other HIFs impeding their transcriptional ability.

Biological function summary

HIF3 alpha engages in cellular response to hypoxic environments by binding to hypoxia-responsive elements in DNA. It forms heterodimers with ARNT an aryl hydrocarbon receptor nuclear translocator allowing interaction with DNA sequences needed for hypoxia response. HIF3 alpha differs by sometimes inhibiting rather than activating target genes providing a balance to the functions of other HIFs. It affects vascular development and energy metabolism performing roles outside traditional hypoxia response.

Pathways

HIF3 alpha integrates into key biological networks such as the HIF signaling pathway and oxygen homeostasis. Within these pathways it interacts with other HIF family members including HIF1 alpha and HIF2 alpha regulating downstream hypoxia-adaptive responses. This interaction allows a fine-tuned balance in the expression of genes linked to angiogenesis and erythropoiesis exemplifying its antagonistic function in comparison to other HIFs that usually activate these pathways.

HIF3 alpha assumes significant roles in the context of conditions like non-small cell lung carcinoma (NSCLC) and sleep apnea. Studies suggest alterations in HIF3 alpha expression contribute to tumor progression in NSCLC and the regulation of respiratory drive under low oxygen conditions in sleep apnea. Through these disease processes potential connections with proteins like VEGF (Vascular Endothelial Growth Factor) underline HIF3 alpha's modulatory impact in angiogenic activity and hypoxic responses affecting disease progression and outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression (PubMed : 11573933, PubMed : 16126907, PubMed : 19694616, PubMed : 20416395, PubMed : 21069422). Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity).. Isoform 2. Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. Also inhibits hypoxia-inducible ARNT-mediated gene expression.. Isoform 3. Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation.. Isoform 4. Attenuates the ability of transcription factor HIF1A and EPAS1/HIF2A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation (PubMed : 16126907, PubMed : 17998805, PubMed : 19694616, PubMed : 20416395). May act as a tumor suppressor and inhibits malignant cell transformation (PubMed : 17998805).. Isoform 5. Attenuates the ability of transcription factor HIF1A to bind to hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation.

See full target information HIF3A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 130:1904-1915 PubMed38693428

2024

Hypoxia-inducible factor 3α1 increases epithelial-to-mesenchymal transition and iron uptake to drive colorectal cancer liver metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Luke B Villareal,Daniel M Falcon,Liwei Xie,Xiang Xue

Evidence-based complementary and alternative medic 2019:2807926 PubMed30941190

2019

Jian-Pi-Yi-Shen Decoction Relieves Renal Anemia in 5/6 Nephrectomized Rats: Production of Erythropoietin via Hypoxia Inducible Factor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Chen,Fochang Wang,Shiying Huang,Xiaoyan Liu,Zhonggui Li,Airong Qi,Xinhui Liu,Tiegang Yi,Shunmin Li

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HIF3 alpha/IPAS antibody

Western blot - Anti-HIF3 alpha/IPAS antibody (AB176464)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise