Anti-HIF3 alpha/IPAS antibody

• WB

Unknown

Western blot - Anti-HIF3 alpha/IPAS antibody (AB2165)

Exponentially growing 3T3 cells were treated with 130μM CoCl₂ for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.

All lanes:

Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution

All lanes:

3T3 lysate treated with Cobalt (II) chloride at 10 µg/mL

Secondary

All lanes:

Goat-anti-Rabbit at 1/10000 dilution

Predicted band size: 72 kDa

Observed band size: 24 kDa,55 kDa,72 kDa

false

• WB

CiteAb

Western blot - Anti-HIF3 alpha/IPAS antibody (AB2165)

HIF3 alpha/IPAS western blot using anti-HIF3 alpha/IPAS antibody ab2165. Publication image and figure legend from Huang, C. & Hales, B. F., 2012, PLoS One, PubMed 23251655.

ab2165 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab2165 please see the product overview.

Western blot of HIF3A.HIF3A protein was detected in control limbs at all time points. A : Western blot analysis of HIF3A protein (118 KD) and actin (43 KD) in limbs at 30 min, 1, 3, 6, and 24 h after treatment with 4-OOHCPA at 0.3 µg/ml (L) 1.0 µg/ml (M) or 3.0 µg/ml (H). B : Densitometric quantification of HIF3A in drug treated groups compared to control groups. Hatched bar : 0.3 µg/ml (L), cross-hatched bar : 1.0 µg/ml (M) and gray bar : 3.0 µg/ml (H). HIF3A protein concentrations were not affected by drug treatment or time. Each bar (mean ± SEM) represents six to seven replicates.

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