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AB117456

Anti-HIGD1A antibody

1

(2 Reviews)

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(2 Publications)

Rabbit Polyclonal HIGD1A antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HIGD1A.

View Alternative Names

HIG1, HSPC010, HIGD1A, Hypoxia-inducible gene 1 protein, RCF1 homolog A, RCF1a

3 Images
Immunocytochemistry - Anti-HIGD1A antibody (AB117456)
  • ICC

Unknown

Immunocytochemistry - Anti-HIGD1A antibody (AB117456)

Immunocytochemical analysis of 293 cells, staining HIGD1A with ab117456 at 2.5 µg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-HIGD1A antibody (AB117456)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIGD1A antibody (AB117456)

Immunofluorescence of HIGD1A in 293 cells tissue with ab117456 at 10 ug/mL.

Western blot - Anti-HIGD1A antibody (AB117456)
  • WB

Unknown

Western blot - Anti-HIGD1A antibody (AB117456)

Lane 1:

Western blot - Anti-HIGD1A antibody (ab117456) at 0.5 µg/mL

Lane 2:

Western blot - Anti-HIGD1A antibody (ab117456) at 1 µg/mL

All lanes:

293 cell lysate at 15 µg

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, ICC

applications

Immunogen

Synthetic Peptide within Human HIGD1A. The exact immunogen used to generate this antibody is proprietary information.

Q9Y241

Specificity

ab117456 is predicted to not cross-react with HIG2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2.5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Avoid repeated freeze thaw cycles and exposure to prolonged high temperatures.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab117456 was purified by affinity chromatography via a peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIGD1A also known as Hypoxia Inducible Gene Domain Family Member 1A functions as a mitochondrial protein. It weighs approximately 12 kDa. It localizes within the inner mitochondrial membrane where it regulates respiratory chain activity. HIGD1A expresses in multiple tissues with high levels in the brain heart and skeletal muscle. It plays a role in cellular responses to hypoxic stress conditions modulating mitochondrial energy production.
Biological function summary

The protein influences cellular survival during low oxygen levels which makes it essential in the physiological adaptation to hypoxia. HIGD1A integrates into the larger mitochondrial complex IV also known as cytochrome c oxidase. By interacting closely with this complex HIGD1A adjusts mitochondrial output and efficiency thereby impacting the generation of reactive oxygen species and ATP in the cell. This association highlights the protein's role in maintaining metabolic balance.

Pathways

HIGD1A participates importantly in the hypoxia signaling pathway and the oxidative phosphorylation pathway. In the hypoxic condition it stabilizes hypoxia-inducible factors (HIFs) that play a central role in adapting cellular metabolism. Furthermore HIGD1A works alongside other proteins like NDUFA4 and COX4I1 key components of the electron transport chain. These connections highlight HIGD1A’s role in energy metabolism and stress response pathways.

The protein establishes a relationship with ischemic conditions and neurodegenerative diseases. It shows altered expression in ischemic cardiac and brain tissues suggesting a protective response to hypoxic injury. Specifically changes in HIGD1A expression levels connect to disorders like Alzheimer's disease. In these conditions the interaction with proteins such as APOE may influence disease progression through mechanisms related to cellular energy metabolism and stress response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes.
See full target information HIGD1A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of advanced research 60:215-231 PubMed37479180

2023

Procyanidin C1 inhibits tumor growth and metastasis in colon cancer via modulating miR-501-3p/HIGD1A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Lin Lv,Yu-Jun Tan,Yu-Shan Ren,Ru Ma,Xiao Wang,Shu-Yan Wang,Wan-Qing Liu,Qiu-Sheng Zheng,Jing-Chun Yao,Jun Tian,Jie Li

Thoracic cancer 14:913-928 PubMed36808485

2023

Delivery of miR-3529-3p using MnO -SiO -APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Ran-Ran Wang,Rui Liu,Shu-Yang Xie,Fei Jiao,You-Jie Li,Jiaxuan Xin,Han Zhang,Zhenbo Wang,Yun-Fei Yan
View all publications
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