AB264376

Anti-HIP1 antibody

Name: Anti-HIP1 antibody (ab264376) | Abcam
Brand: Abcam Limited
SKU: AB264376
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal HIP1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HIP1 aa 700-800.

View Alternative Names

Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein I, HIP-I, HIP1

2 Images

Supplier Data

Western blot - Anti-HIP1 antibody (AB264376)

Lysates prepared in NETN lysis buffer.

All lanes:

Western blot - Anti-HIP1 antibody (ab264376) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 116 kDa

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Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-HIP1 antibody (AB264376)

HIP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264376 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264376 at 1 μg/ml.

Lane 1 : ab264376 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 30 seconds.

Lysates prepared in NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-HIP1 antibody (ab264376)

Predicted band size: 116 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human HIP1 aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

O00291

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP1 also known as Huntingtin-interacting protein 1 is a protein with a mass of approximately 116 kDa. HIP1 is an adapter protein involved in the endocytosis process. It interacts with clathrin and AP2 adaptor complex assisting in the invagination and fission of clathrin-coated pits. HIP1 is present throughout various tissues including the brain and immune cells indicating its widespread role in cellular mechanisms.

Biological function summary

HIP1 plays an essential role in the modulation of receptor-mediated endocytosis. It is part of a larger complex with epsin and other proteins facilitating the internalization of extracellular signals. The protein's interaction with actin and lipid membranes suggests its function in reorganizing cytoskeleton arrangements vital for cell movement and shape changes. Cellular homeostasis and signal transduction rely on HIP1's ability to mediate these activities.

Pathways

HIP1 integrates into the PI3K/AKT signaling impacting cell survival and apoptosis. Its activity in clathrin-mediated endocytosis also links it to the receptor tyrosine kinase (RTK) pathway. In these pathways HIP1 assists in the regulation of growth factor receptors collaborating with proteins such as GRB2 and SHC1. These interactions ensure proper cellular responses to external stimuli.

HIP1 has associations with cancer and neurodegenerative diseases. In cancer overexpression of HIP1 enhances cell proliferation linked to its role in receptor trafficking and signaling. HIP1's interaction with huntingtin the protein mutated in Huntington's disease suggests its involvement in neurodegeneration. Such connections emphasize the protein's potential as a target for therapeutic intervention highlighting HIP1's impact on pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in clathrin-mediated endocytosis and trafficking (PubMed : 11532990, PubMed : 11577110, PubMed : 11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity). Regulates presynaptic nerve terminal activity (By similarity). Enhances androgen receptor (AR)-mediated transcription (PubMed : 16027218). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed : 11007801). Binds 3-phosphoinositides (via ENTH domain) (PubMed : 14732715). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed : 14732715). May play a functional role in the cell filament networks (PubMed : 18790740). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed : 11007801, PubMed : 12163454).

See full target information HIP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HIP1 antibody

Western blot - Anti-HIP1 antibody (AB264376)

Immunoprecipitation - Anti-HIP1 antibody (AB264376)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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