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AB181238

Anti-HIP1 antibody [EPR10814]

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(3 Publications)

Rabbit Recombinant Monoclonal HIP1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein I, HIP-I, HIP1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HIP1 antibody [EPR10814] (AB181238)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HIP1 antibody [EPR10814] (AB181238)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling HIP1 with ab181238 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunoprecipitation - Anti-HIP1 antibody [EPR10814] (AB181238)
  • IP

Supplier Data

Immunoprecipitation - Anti-HIP1 antibody [EPR10814] (AB181238)

Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181238 at 1/50 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-HIP1 antibody [EPR10814] (ab181238)

Predicted band size: 116 kDa

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Western blot - Anti-HIP1 antibody [EPR10814] (AB181238)
  • WB

Lab

Western blot - Anti-HIP1 antibody [EPR10814] (AB181238)

Lanes 1 - 3 : Merged signal (red and green). Green - ab181238 observed at 116 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab181238 was shown to react with HIP1 in wild-type HAP1 cells in western blot with loss of signal observed in HIP1 knockout sample. Wild-type and HIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181238 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HIP1 antibody [EPR10814] (ab181238) at 1/1000 dilution

Lane 1:

wild-type HAP1 cell lysate at 20 µg

Lane 2:

HIP1 knockout HAP1 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 116 kDa

Observed band size: 116 kDa

false

Western blot - Anti-HIP1 antibody [EPR10814] (AB181238)
  • WB

Supplier Data

Western blot - Anti-HIP1 antibody [EPR10814] (AB181238)

All lanes:

Western blot - Anti-HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution

Lane 1:

HCT-116 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa

false

  • Carrier free

    Anti-HIP1 antibody [EPR10814] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10814

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP1 also known as Huntingtin-interacting protein 1 is a protein with a mass of approximately 116 kDa. HIP1 is an adapter protein involved in the endocytosis process. It interacts with clathrin and AP2 adaptor complex assisting in the invagination and fission of clathrin-coated pits. HIP1 is present throughout various tissues including the brain and immune cells indicating its widespread role in cellular mechanisms.
Biological function summary

HIP1 plays an essential role in the modulation of receptor-mediated endocytosis. It is part of a larger complex with epsin and other proteins facilitating the internalization of extracellular signals. The protein's interaction with actin and lipid membranes suggests its function in reorganizing cytoskeleton arrangements vital for cell movement and shape changes. Cellular homeostasis and signal transduction rely on HIP1's ability to mediate these activities.

Pathways

HIP1 integrates into the PI3K/AKT signaling impacting cell survival and apoptosis. Its activity in clathrin-mediated endocytosis also links it to the receptor tyrosine kinase (RTK) pathway. In these pathways HIP1 assists in the regulation of growth factor receptors collaborating with proteins such as GRB2 and SHC1. These interactions ensure proper cellular responses to external stimuli.

HIP1 has associations with cancer and neurodegenerative diseases. In cancer overexpression of HIP1 enhances cell proliferation linked to its role in receptor trafficking and signaling. HIP1's interaction with huntingtin the protein mutated in Huntington's disease suggests its involvement in neurodegeneration. Such connections emphasize the protein's potential as a target for therapeutic intervention highlighting HIP1's impact on pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in clathrin-mediated endocytosis and trafficking (PubMed : 11532990, PubMed : 11577110, PubMed : 11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (By similarity). Regulates presynaptic nerve terminal activity (By similarity). Enhances androgen receptor (AR)-mediated transcription (PubMed : 16027218). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (PubMed : 11007801). Binds 3-phosphoinositides (via ENTH domain) (PubMed : 14732715). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (PubMed : 14732715). May play a functional role in the cell filament networks (PubMed : 18790740). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed : 11007801, PubMed : 12163454).
See full target information HIP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabl5032 PubMed35613266

2022

Selective endocytosis of Ca-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Azarnia Tehran,Gaga Kochlamazashvili,Niccolò P Pampaloni,Silvia Sposini,Jasmeet Kaur Shergill,Martin Lehmann,Natalya Pashkova,Claudia Schmidt,Delia Löwe,Hanna Napieczynska,Arnd Heuser,Andrew J R Plested,David Perrais,Robert C Piper,Volker Haucke,Tanja Maritzen

Neuropathology and applied neurobiology 47:640-652 PubMed33368549

2021

MOBP and HIP1 in multiple system atrophy: New α-synuclein partners in glial cytoplasmic inclusions implicated in the disease pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Conceição Bettencourt,Yasuo Miki,Ignazio S Piras,Rohan de Silva,Sandrine C Foti,Joshua S Talboom,Tamas Revesz,Tammaryn Lashley,Robert Balazs,Emmanuelle Viré,Thomas T Warner,Matt J Huentelman,Janice L Holton

Frontiers in molecular neuroscience 11:447 PubMed30574069

2018

Endocytic Adaptor Protein HIP1R Controls Intracellular Trafficking of Epidermal Growth Factor Receptor in Neuronal Dendritic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Yang,Lin Peng,Yu Wu,Yanan Li,Ling Wang,Jian-Hong Luo,Junyu Xu
View all publications
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