AB140608

Anti-HIP1R antibody [EPR9437]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal HIP1R antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

HIP12, KIAA0655, HIP1R, Huntingtin-interacting protein 1-related protein, HIP1-related protein, Huntingtin-interacting protein 12, HIP-12

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] (AB140608)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunofluorescent analysis of HeLa cells labelling HIP1R with ab140608 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab140608 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HIP1R with ab140608 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] (AB140608)

Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HIP1R antibody [EPR9437] (AB140608)

WB

Unknown

Western blot - Anti-HIP1R antibody [EPR9437] (AB140608)

All lanes:

Western blot - Anti-HIP1R antibody [EPR9437] (ab140608) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 119 kDa

false

Carrier free

Anti-HIP1R antibody [EPR9437] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9437

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP1R also known as Huntingtin-interacting protein 1 related is an endocytic adaptor protein. This protein weighs approximately 116 kDa. It primarily facilitates clathrin-mediated endocytosis by linking endocytic vesicles to the actin cytoskeleton. HIP1R is enriched in neuronal tissues particularly within the brain and is present in other tissues like muscle and liver. The protein contains a domain structure having an ENTH domain and coiled-coil regions which allow it to interact with other cellular components.

Biological function summary

HIP1R contributes to membrane trafficking and actin dynamics. As part of the endocytic machinery HIP1R directly interacts with clathrin and actin fostering vesicle motility and shaping membrane curvature. The protein resides within a larger protein complex that includes clathrin actin and other endocytic proteins. Its role in vesicle formation and transport is essential for maintaining cellular homeostasis by controlling receptor internalization and sorting.

Pathways

HIP1R participates in the clathrin-mediated endocytosis and actin cytoskeleton regulation pathways. It shares functional connections with proteins such as clathrin and actin emphasizing its role in vesicular dynamics and membrane trafficking. The protein ensures effective communication and transport within cells by coordinating with these pathways. These processes contribute to essential cellular functions like nutrient uptake and signal transduction.

HIP1R associates with neurodegenerative conditions and cancer. Altered expression of HIP1R can occur in pathological states impacting endocytic and cytoskeletal functions. Its interaction with Huntingtin another significant protein connects it to Huntington’s disease where proper endocytic action becomes disrupted. In cancer dysregulation of endocytosis-related proteins including HIP1R leads to aberrant cell signaling and proliferation linking HIP1R to oncogenic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

See full target information HIP1R

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

MedComm 6:e70138 PubMed40264576

2025

Integrating Multiplex Immunohistochemistry and Machine Learning for Glioma Subtyping and Prognosis Prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Houshi Xu,Zhen Fan,Shan Jiang,Maoyuan Sun,Huihui Chai,Ruize Zhu,Xiaoyu Liu,Yue Wang,Jiawen Chen,Junji Wei,Ying Mao,Zhifeng Shi

Cancers 15: PubMed36612024

2022

Syntaxin-6, a Reliable Biomarker for Predicting the Prognosis of Patients with Cancer and the Effectiveness of Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchao Li,Kuan Li,Hongfa Wei,Yu Sun,Yangjing Liao,Yuan Zou,Xiancong Chen,Cuncan Deng,Songyao Chen,Yulong He,Mingyu Huo,Changhua Zhang

Neuro-oncology 24:2121-2132 PubMed35511748

2022

HIP1R and vimentin immunohistochemistry predict 1p/19q status in IDH-mutant glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Felix,Dennis Friedel,Ashok Kumar Jayavelu,Katharina Filipski,Annekathrin Reinhardt,Uwe Warnken,Damian Stichel,Daniel Schrimpf,Andrey Korshunov,Yueting Wang,Tobias Kessler,Nima Etminan,Andreas Unterberg,Christel Herold-Mende,Laura Heikaus,Felix Sahm,Wolfgang Wick,Patrick N Harter,Andreas von Deimling,David E Reuss

European journal of medical research 27:29 PubMed35209947

2022

Flurbiprofen inhibits cell proliferation in thyroid cancer through interrupting HIP1R-induced endocytosis of PTEN.

Applications

Unspecified application

Species

Unspecified reactive species

Nengli Yang,Yafeng Liang,Pei Yang,Liuming Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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