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AB248899

Anti-HIP1R antibody [EPR9437] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HIP1R antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HIP12, KIAA0655, HIP1R, Huntingtin-interacting protein 1-related protein, HIP1-related protein, Huntingtin-interacting protein 12, HIP-12

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling HIP1R with ab140608 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HIP1R with ab140608 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab140608 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab140608 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)
  • WB

Unknown

Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)

This data was developed using ab140608, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HIP1R antibody [EPR9437] (<a href='/en-us/products/primary-antibodies/hip1r-antibody-epr9437-ab140608'>ab140608</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 119 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9437

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248899 is the carrier-free version of ab140608.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP1R also known as Huntingtin-interacting protein 1 related is an endocytic adaptor protein. This protein weighs approximately 116 kDa. It primarily facilitates clathrin-mediated endocytosis by linking endocytic vesicles to the actin cytoskeleton. HIP1R is enriched in neuronal tissues particularly within the brain and is present in other tissues like muscle and liver. The protein contains a domain structure having an ENTH domain and coiled-coil regions which allow it to interact with other cellular components.
Biological function summary

HIP1R contributes to membrane trafficking and actin dynamics. As part of the endocytic machinery HIP1R directly interacts with clathrin and actin fostering vesicle motility and shaping membrane curvature. The protein resides within a larger protein complex that includes clathrin actin and other endocytic proteins. Its role in vesicle formation and transport is essential for maintaining cellular homeostasis by controlling receptor internalization and sorting.

Pathways

HIP1R participates in the clathrin-mediated endocytosis and actin cytoskeleton regulation pathways. It shares functional connections with proteins such as clathrin and actin emphasizing its role in vesicular dynamics and membrane trafficking. The protein ensures effective communication and transport within cells by coordinating with these pathways. These processes contribute to essential cellular functions like nutrient uptake and signal transduction.

HIP1R associates with neurodegenerative conditions and cancer. Altered expression of HIP1R can occur in pathological states impacting endocytic and cytoskeletal functions. Its interaction with Huntingtin another significant protein connects it to Huntington’s disease where proper endocytic action becomes disrupted. In cancer dysregulation of endocytosis-related proteins including HIP1R leads to aberrant cell signaling and proliferation linking HIP1R to oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
See full target information HIP1R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com