Rabbit Recombinant Monoclonal HIP1R antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--western-blot-img77489.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)")
![Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunocytochemistry-immunofluorescence-img129208.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117975.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131732.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (AB248899)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Alternative names
Huntingtin-interacting protein 1-related protein, HIP1-related protein, Huntingtin-interacting protein 12, HIP-12, HIP1R, HIP12, KIAA0655
Recommended products
Rabbit Recombinant Monoclonal HIP1R antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Alternative names
Huntingtin-interacting protein 1-related protein, HIP1-related protein, Huntingtin-interacting protein 12, HIP-12, HIP1R, HIP12, KIAA0655
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR9437
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab248899 is the carrier-free version of Anti-HIP1R antibody [EPR9437] ab140608.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HIP1R also known as Huntingtin-interacting protein 1 related is an endocytic adaptor protein. This protein weighs approximately 116 kDa. It primarily facilitates clathrin-mediated endocytosis by linking endocytic vesicles to the actin cytoskeleton. HIP1R is enriched in neuronal tissues particularly within the brain and is present in other tissues like muscle and liver. The protein contains a domain structure having an ENTH domain and coiled-coil regions which allow it to interact with other cellular components.
- Biological function summary
HIP1R contributes to membrane trafficking and actin dynamics. As part of the endocytic machinery HIP1R directly interacts with clathrin and actin fostering vesicle motility and shaping membrane curvature. The protein resides within a larger protein complex that includes clathrin actin and other endocytic proteins. Its role in vesicle formation and transport is essential for maintaining cellular homeostasis by controlling receptor internalization and sorting.
- Pathways
HIP1R participates in the clathrin-mediated endocytosis and actin cytoskeleton regulation pathways. It shares functional connections with proteins such as clathrin and actin emphasizing its role in vesicular dynamics and membrane trafficking. The protein ensures effective communication and transport within cells by coordinating with these pathways. These processes contribute to essential cellular functions like nutrient uptake and signal transduction.
- Associated diseases and disorders
HIP1R associates with neurodegenerative conditions and cancer. Altered expression of HIP1R can occur in pathological states impacting endocytic and cytoskeletal functions. Its interaction with Huntingtin another significant protein connects it to Huntington’s disease where proper endocytic action becomes disrupted. In cancer dysregulation of endocytosis-related proteins including HIP1R leads to aberrant cell signaling and proliferation linking HIP1R to oncogenic pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--western-blot-img77489.jpg "Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Western blot - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HIP1R antibody [EPR9437] (Anti-HIP1R antibody [EPR9437] ab140608) at 1/1000 dilution
Lane 1: Human fetal kidney lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 119 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136975.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HIP1R with Anti-HIP1R antibody [EPR9437] ab140608 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunocytochemistry-immunofluorescence-img129208.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunocytochemistry/ Immunofluorescence - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling HIP1R with Anti-HIP1R antibody [EPR9437] ab140608 at 1/100 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117975.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Hepatocellular carcinoma tissue using Anti-HIP1R antibody [EPR9437] ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131732.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using Anti-HIP1R antibody [EPR9437] ab140608 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96503.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using Anti-HIP1R antibody [EPR9437] ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135065.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)
This data was developed using Anti-HIP1R antibody [EPR9437] ab140608, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using Anti-HIP1R antibody [EPR9437] ab140608 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96503.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899), expandable thumbnail](https://content.abcam.com/products/images/hip1r-antibody-epr9437-bsa-and-azide-free-ab248899--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135065.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP1R antibody [EPR9437] - BSA and Azide free (ab248899)")
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